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  6. Thermo Scientific™ CaptureSelect™ HRP Anti-AAVX Conjugate

Thermo Scientific™ CaptureSelect™ HRP Anti-AAVX Conjugate
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Catalog No. 7303522500
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Catalog No. 7303522500 Supplier Thermo Scientific™ Supplier No. 7303522500

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Thermo Scientific™ CaptureSelect™ HRP Anti-AAVX Conjugate includes a 14-kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds adeno-associated virus particles of multiple AAV serotypes.

CaptureSelect HRP Anti-AAVX Conjugate includes a 14-kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds adeno-associated virus particles of multiple AAV serotypes. The antibody fragment binds with high affinity to AAV particles and demonstrates good binding reactivity towards a variety of AAV serotypes, including AAV1 to AAV8, and AAVrh10. The antibody fragment is chemically conjugated to horseradish peroxidase (HRP) via a spacer of appropriate length to maintain full binding capacity of the antibody fragment.

CaptureSelect HRP Anti-AAVX Conjugate can be combined with CaptureSelect Biotin Anti-AAVX Conjugate (Cat. No. 7103522100) in enzyme linked immunoassays to quantify the total number of AAV capsids. Sandwich ELISAs can be designed using the biotin anti-AAVX conjugate as a capture reagent immobilized on a streptavidin coated surface. HRP anti-AAVX conjugate is then used for detection of the bound AAV capsids. The unique binding properties of this pair of anti-AAVX conjugates can circumvent the need for incorporation of different AAV serotype-specific antibodies for AAV assay development.

For assay development to measure AAV9 total capsids, we recommend CaptureSelect Biotin Anti-AAV9 Conjugate (Cat. No. 7103332100) and CaptureSelect HRP Anti-AAV9 Conjugate (Cat. No. 7303332100).

Product details

  • Format type: HRP, liquid
  • Buffer: PBS, 50% (v/v) glycerol
  • Storage: in freezer (-5 to -30°C)
  • Concentration: 0.5 mg/mL
  • Volume: 0.1 mL
  • Recommended working dilution for sandwich ELISA: 1: 5000 to 1: 10,000 (the optimal working dilution should be determined empirically for each application)

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Specifications

Concentration 0.5 mg/mL
Content And Storage Store in freezer (-5°C to -30°C).
Form Liquid
Product Line CaptureSelect
Quantity 500 μL
For Use With (Application) Quantitation of multiple AAV capsids
Type Conjugate