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Invitrogen™ eBioscience™ Calcein Blue AM Viability Dye
Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Blue.
Supplier: Invitrogen™ 65085539
Description
- Molecular weight: 465.41g/mol
- Peak excitation: 360nm
- Peak emission: 445nm
- Reconstitute in high-quality, freshly opened DMSO
- Once reconstituted, store at −20°C with dessicant and use within a short period of time. Avoid freeze-thawing
- Calcein Blue is optimally excited by the UV laser line (360nm), but can also be excited by the violet laser line (405nm)
- For fluorescent microscopy, detect using the appropriate filter sets
- Calcein Blue AM is itself weakly fluorescent, and an additional wash step may be necessary to reduce background fluorescence
- Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells
Specifications
| Blue | |
| Viability Assay | |
| Dye | |
| eBioscience |
| Fluorescence | |
| Fluorescence Microscope, Flow Cytometer | |
| 408/450 | |
| 1 mg |
For Research Use Only. Not for use in diagnostic procedures.