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  6. Invitrogen™ BrightComp eBeads™ Compensation Bead Kits

Invitrogen™ BrightComp eBeads™ Compensation Bead Kits

Catalog No. A54740
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Description:
GFP BrightComp eBeads Compensation Bead Kit
mCherry BrightComp eBeads Compensation Bead Kit
RFP BrightComp eBeads Compensation Bead Kit
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Catalog No. Description
A54740 RFP BrightComp eBeads Compensation Bead Kit
A54743 mCherry BrightComp eBeads Compensation Bead Kit
A10514 GFP BrightComp eBeads Compensation Bead Kit
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Catalog No. A54740 Supplier Invitrogen™ Supplier No. A54740
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Description

BrightComp eBeads compensation beads provide a consistent, accurate, and simple-to-use technique for setting flow cytometry compensation when using fluorescent protein-expressing samples.

• Three levels of intensity, perfect for any samples no matter the level of expression
• Near-identical, spectrally-matched dye to fluorescent protein
• Consistent, reliable performance

Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Each kit provides polystyrene microspheres with near-identical, spectrally-matched fluorescent protein dye as well as negative control beads in a single dropper bottle.

Specifications

Description RFP BrightComp eBeads Compensation Bead Kit
Quantity 25 tests
Excitation Wavelength Range 561 nm
For Use With (Application) Flow Cytometry
Product Type Compensation Beads

Frequently Asked Questions (FAQs)

Can I use the GFP BrightComp eBeads to compensate for FITC or Alexa Fluor 488?

No. You must always compensate with the same fluorophore present on the samples. The GFP BrightComp eBeads Compensation Bead Kit spectrally emulates GFP and must only be used on GFP-expressing samples.
I used the GFP BrightComp eBeads Compensation Bead Kit. My peaks are broader than what I see in the manual (Figure 1). What is causing this?

This may be caused by aggregation of the beads. Mix thoroughly before dispensing a drop from the stock vial and mix again after adding buffer. Aggregation may be also be caused by microbial contamination or if the beads were frozen. In these cases, they can no longer be used.
What material are the beads in the GFP BrightComp eBeads Compensation Bead Kit composed of?

These are polystyrene beads.