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Invitrogen™ BLOCK-iT™ Fluorescent Oligo, for electroporation

Catalog No. 13750062
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75 μL
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13750062 75 μL
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Catalog No. 13750062 Supplier Invitrogen™ Supplier No. 13750062
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Description

BLOCK-iT™ Fluorescent Oligo, for electroporation

BLOCK-iT™ Fluorescent Oligo provides:

  • A strong, easily detectable FITC signal indicating transfection efficiency
  • Chemical modifications for a clearer, more persistent cellular signal than labeled siRNA
  • Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA molecules

    This makes the BLOCK-iT™ Fluorescent Oligo an ideal indicator of transfection efficiency for RNAi experiments. BLOCK-iT™ Fluorescent Oligo is available in two configurations: one designed for lipid-mediated transfection (2 x 125μL) at 20μM and one designed for electro-poration (75μl) at 1 mM.

    • Specifications

    Content And Storage The BLOCK-iT™ Fluorescent Oligo for electroporation is supplied as 75 μl at 1 mM, and should be stored at -20° C.
    Control Type Transfection Control
    Detection Method Fluorescence
    Sample Type RNA
    For Use With (Application) Electroporation
    Label or Dye FITC (Fluorescein)
    Product Line BLOCK-iT
    Product Type Fluorescent Oligo
    Quantity 75 μL
    RNAi Type siRNA

    Frequently Asked Questions (FAQs)

    What are the positive and negative controls I should use with my Stealth RNAi siRNA experiment?

    We offer several negative and positive controls for Stealth siRNA:

    For Stealth Positive Control:
    - Stealth RNAi siRNA GAPDH Positive Control (human) (Cat. No. 12935140, 250 µL)
    - Stealth RNAi siRNA beta-Lactamase Reporter Control (Cat. No. 12935148, 250 µL)
    - Stealth RNAi siRNA GFP Reporter Control (Cat. No. 12935145, 250 µL)
    - Stealth RNAi siRNA Luciferase Reporter Control (Cat. No. 12935146, 250 µL)

    For Stealth Negative Control:
    Hi (55-65% GC), Med (45-55% GC), or Low (35-45% GC) Duplexes are offered:

    - Stealth RNAi siRNA Negative Control Hi GC (Cat. No. 12935400, 250 µL)
    - Stealth RNAi siRNA Negative Control Hi GC Duplex #2 (Cat. No. 12935114, 250 µL)
    - Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935115, 250 µL)
    - Stealth RNAi siRNA Negative Control Med GC Duplex (Cat. No. 12935300, 250 µL)
    - Stealth RNAi siRNA Negative Control Med GC Duplex #2 (Cat. No. 12935112, 250 µL)
    - Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935113, 250 µL)
    - Stealth RNAi siRNA Negative Control Low GC Duplex (Cat. No. 12935200, 250 µL)
    - Stealth RNAi siRNA Negative Control Low GC Duplex #2 (Cat. No. 12935110, 250 µL)
    - Stealth RNAi siRNA Negative Control Low GC Duplex #3 (Cat. No. 12935111, 250 µL)
    - Stealth RNAi siRNA Negative Control Kit (Cat. No. 12935100, contains one of each GC content level negative control, 250 µL each at 20 µM)
    - For a scrambled negative control, you could also scramble your designed siRNA or Stealth RNAi sequence using the BLOCK-iT RNAi Designer.

    You can also use our BLOCK-iT AlexaFluor Red Fluorescent Control (Cat. No. 14750100) or BLOCK-iT Fluorescent Oligo (Cat. No. 2013, 13750062) as a control for the transfection.

    For Research Use Only. Not for use in diagnostic procedures.