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Invitrogen™ BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control

Catalog No. 14750100
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Catalog No. 14750100 Supplier Invitrogen™ Supplier No. 14750100
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Description

BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control

The BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control is an ideal indicator of lipid-mediated transfection efficiency for RNAi experiments, particularly those using the Lipofectamine™ RNAiMAX Transfection Reagent. The sequence of the BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control is not homologous to any known gene, preventing induction of nonspecific cellular events caused by introduction of the duplex into cells. The BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control provides:
  • A strong, easily detectable, and photostable Alexa Fluor™ 555* signal indicating transfection efficiency
  • Chemical modifications for a clearer, more persistent cellular signal than with labeled siRNA
  • Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA molecules *This dye can be detected using standard filter sets designed for Alexa Fluor™ 555, Cy™3, DsRed, Texas Red™, or rhodamine fluorophores.

    • Specifications

    Content And Storage The BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control is provided at a concentration of 20 μM. Store at -20°C. Guaranteed stable for 12 months when properly stored.
    Control Type Transfection Control
    Detection Method Fluorescence
    Sample Type RNA
    For Use With (Application) RNA Transfection
    Label or Dye Alexa Fluor 555
    Product Line Alexa Fluor, BLOCK-iT
    Product Type Red fluorescent transfection control
    Quantity 2 x 125 μL
    RNAi Type siRNA
    Shipping Condition Dry Ice
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    Frequently Asked Questions (FAQs)

    What are the positive and negative controls I should use with my Stealth RNAi siRNA experiment?

    We offer several negative and positive controls for Stealth siRNA:

    For Stealth Positive Control:
    - Stealth RNAi siRNA GAPDH Positive Control (human) (Cat. No. 12935140, 250 µL)
    - Stealth RNAi siRNA beta-Lactamase Reporter Control (Cat. No. 12935148, 250 µL)
    - Stealth RNAi siRNA GFP Reporter Control (Cat. No. 12935145, 250 µL)
    - Stealth RNAi siRNA Luciferase Reporter Control (Cat. No. 12935146, 250 µL)

    For Stealth Negative Control:
    Hi (55-65% GC), Med (45-55% GC), or Low (35-45% GC) Duplexes are offered:

    - Stealth RNAi siRNA Negative Control Hi GC (Cat. No. 12935400, 250 µL)
    - Stealth RNAi siRNA Negative Control Hi GC Duplex #2 (Cat. No. 12935114, 250 µL)
    - Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935115, 250 µL)
    - Stealth RNAi siRNA Negative Control Med GC Duplex (Cat. No. 12935300, 250 µL)
    - Stealth RNAi siRNA Negative Control Med GC Duplex #2 (Cat. No. 12935112, 250 µL)
    - Stealth RNAi siRNA Negative Control Hi GC Duplex #3 (Cat. No. 12935113, 250 µL)
    - Stealth RNAi siRNA Negative Control Low GC Duplex (Cat. No. 12935200, 250 µL)
    - Stealth RNAi siRNA Negative Control Low GC Duplex #2 (Cat. No. 12935110, 250 µL)
    - Stealth RNAi siRNA Negative Control Low GC Duplex #3 (Cat. No. 12935111, 250 µL)
    - Stealth RNAi siRNA Negative Control Kit (Cat. No. 12935100, contains one of each GC content level negative control, 250 µL each at 20 µM)
    - For a scrambled negative control, you could also scramble your designed siRNA or Stealth RNAi sequence using the BLOCK-iT RNAi Designer.

    You can also use our BLOCK-iT AlexaFluor Red Fluorescent Control (Cat. No. 14750100) or BLOCK-iT Fluorescent Oligo (Cat. No. 2013, 13750062) as a control for the transfection.

    Can I use the BLOCK-iT Fluorescent Oligo, for lipid transfection (Cat. No. 2013) to assess transfection efficiency with Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778100)?

  • We do not recommend using BLOCK-iT Fluorescent Oligo, for lipid transfection (Cat. No. 2013) to assess transfection efficiency with Lipofectamine RNAiMAX Transfection Reagent (Cat. No. 13778100). BLOCK-iT Alexa Fluor Red Fluorescent Control (Cat. No. 14750100) should be used to assess transfection efficiency with Lipofectamine RNAiMAX Reagent.
  • BLOCK-iT Fluorescent Oligo, for lipid transfection is optimized for use with Lipofectamine 2000 Transfection Reagent and is not recommended for Lipofectamine RNAiMAX Reagent.

    • For Research Use Only. Not for use in diagnostic procedures.