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  6. Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit

Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit

Catalog No. 4458688
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Catalog No. No. of Reactions
4458688 1000 Reactions
4458687 100 Reactions
4458689 24 Reactions
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Catalog No. 4458688 Supplier Applied Biosystems™ Supplier No. 4458688
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Description

Includes

This kit contains BigDye™ Direct PCR Master Mix, Sequencing Master Mix, M13 Fwd Primer, M13 Rev Primer, and Control DNA CEPH 1347-02, This kit contains sufficient reagents for 24 reactions

The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.

  • A streamlined, simple procedure
  • Performance of all sequencing steps in a single reaction tube
  • Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
  • Substantial time savings versus previous workflows

Order Info

1 Kit

Specifications

Content And Storage This kit contains BigDye™ Direct PCR Master Mix, Sequencing Master Mix, M13 Fwd Primer, M13 Rev Primer, and Control DNA CEPH 1347-02. Store all components at -15 to -25°C and protect from light. PCR Master Mix should be stored at 4°C after first use. This kit contains sufficient reagents for 1000 reactions.
Format 8-well Strip, 96-well Plate
For Use With (Equipment) 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, SeqStudio Genetic Analyzer, Veriti Thermal Cycler, 3130 Genetic Analyzer, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer
For Use With (Application) Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes)
Includes BigDye™ Direct Cycle Sequencing Kit, 1000 rxns, 8-well Strips, 96-well Plate, Fluorescent Dye Terminator Sequencing Technique, 3, Use with 130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, GeneAmp™ 9700, Veriti™ Thermal Cycler
No. of Reactions 1000 Reactions
Product Line BigDye
Product Type Direct Cycle Sequencing Kit
Quantity 1,000 reactions
Shipping Condition Dry Ice
Technique Fluorescent Dye Terminator Sequencing
Template Compatibility BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes)
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For Research Use Only. Not for use in diagnostic procedures.