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Gibco™ BG-11 Media, optimized for cyanobacteria
Description
Gibco™ BG-11 Media is optimized for the growth and maintenance of select Cyanobacteria including Synechococcus elongates. The formulation comes ready-to-use at 1X to let you avoid laborious media preparation steps.
Each Gibco™ BG-11 Media comes in the award winning Gibco™ bottle designed for easier use in the biosafety cabinet, minimizing the risk of contamination, and helps you perform cell culture more consistently. Together, the superior packaging and quality, greater reliability, and improved consistency in Cyanobacteria culture, results in better overall efficiency and more robust data (Fig. 1, 2).
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Specifications
Specifications
| Content And Storage | Gibco™ BG-11 Growth Media contains: • 1 L Gibco™ BG-11 Media — Store at 4 °C |
| Form | Liquid |
| Media Type | Algal Media |
| Preparation Method | None (Ready-to-Use) |
| Product Type | Microbiology Media |
| Target Organism Class | Cyanobacteria |
| Quantity | 1 L |
| Shipping Condition | Room Temperature |
Frequently Asked Questions (FAQs)
The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter for pSyn_6, on the other hand, is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving the high level expression of your gene of interest.
The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter was taken from the solanesyl diphosphate synthase gene from Synechocystis sp. Strain PCC 6803. Note: Use of this weak constitutive promoter is a good fit for applications that are hindered by strong expression, such as pathway engineering or complementation of mutant genes normally expressed at low levels (Simkovsky et al., 2012).
The promoter for pSyn_6 is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving high-level expression of your gene of interest. The pSyn-6 vector also includes a ribosome-binding site (GAAGGAG) for efficient initiation of translation, as well as a stop codon. A 6xHis TEV and a V5-His epitope tag is also included in the vector backbone for detection/purification of the gene of interest. NS1 (neutral site 1) homologous recombination sites are present in both vectors for the integration of the vector into the Synechococcus elongatus genome.
After 6 months of storage, the Synechococcus did not lose their competency. We have not yet tested longer storage points.
A double homologous recombination event occurs between the Neutral Site 1 in the genome and between NSa and NSb in the plasmid. This results in the gene of interest and spectinomycin resistance integrating into the genome within the Neutral Site 1.
We no longer offer any strains. Our reagents and protocols were developed using Synechococcus elongatus strain PCC 7942, a model cyanobacterium.