Invitrogen™ beta Galactosidase Polyclonal Antibody

Description

The E. coli gene lacZ encoding beta-galactosidase is an important reporter gene for detecting the expression of recombinant genes in animal cells. In situations where enzymatic detection of beta-galactosidase is not practical, anti-beta-galactosidase antibody has been widely used. Anti-beta-galactosidase antibody can also be used for purification of fusion proteins by immunoprecipitation or immunoadsorption. Furthermore, anti-beta-galactosidase antibody has been shown to be the preferred analytical tool for light microscopic evaluation of lacZ gene transfer, and the beta-galactosidase/anti-beta-galactosidase complex has been used as a model to assess the feasibility of a high-pressure immunodesorption process. This antibody, raised in rabbit against E. coli beta-galactosidase, demonstrates high specificity for the enzyme and is suitable for immunological methods that require high titer and specificity such as immunoblotting (western or dot blot), ELISA, immunoprecipitation, and immunohistochemistry. The appropriate dilution of antibody for a particular application should be determined empirically. We suggest trying dilutions of about 1:5,000 to 1:50,000 for your initial experiments.

Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene have been associated with GM1-gangliosidosis and Morquio B syndrome. Diseases associated with GLB1 include Gm1-Gangliosidosis, Type I and Gm1-Gangliosidosis, Type Ii.