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Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I or Buffer II 
AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.
Supplier: Applied Biosystems™ N8080172
Description
AmpliTaq DNA Polymerase is a 94-kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process, and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial and lot-to-lot.
AmpliTaq DNA Polymerase is available with GeneAmp10X PCR Buffer I or with GeneAmp 10X PCR Buffer II and MgCl2 Solution.
Reliable and robust PCR
The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ∼40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications.
Features
- The most thoroughly characterized enzyme available for the PCR process
- Excellent thermal activity profile makes it good for PCR applications
- QC-tested to guarantee reproducible results
Notes
- For superior PCR performance, we recommend DreamTaq DNA Polymerase.
Specifications
| 5 U/μL | |
| Stand-alone enzyme | |
| Standard | |
| DNA Polymerase | |
| Dry Ice | |
| 1X | |
| 800 Reactions | |
| Separate Components | |
| DNA |
| • AmpliTaq DNA Polymerase (5 U/μL), 200 μL • GeneAmp 10X PCR Buffer II, 4 x 1.5 mL • 25 mM MgCl2, 4 x 1.5 mL Store at -20°C. |
|
| AmpliTaq DNA Polymerase | |
| 5' - 3' | |
| 1000 Units | |
| Standard PCR | |
| No | |
| 3'-A | |
| 5 kb or less | |
| Buffer II |
For Research Use Only. Not for use in diagnostic procedures.