Antibody;100UG;IgG1/kappa;Recognizes Cetuximab specifically, no cross reactivity with other humanized antibodies.;Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Cetuximab F(ab')2.; ;Anti-Cetuximab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development. Immobilized cetuximab at 1 g/mL, add increasing concentrations of Anti-Cetuximab Antibodies (Cat. No. CEB-Y27, 10% human serum) and then add biotinylated cetuximab at 2 g/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 1.2 ng/mL.;Demonstration of the specificity of Anti-Cetuximab Antibodies (Cat. No. CEB-Y27) to the cetuximab.;Anti-Cetuximab Antibodies (mouse IgG1, Cat. No. CEB-Y27) captured on CM5 chip via anti-mouse antibodies surface, can bind Human cetuximab with an affinity constant of 7.28 pM.
