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Cytiva Amersham™ ECL™ Western Blotting System
Detect mouse and rabbit primary antibodies on Western blots. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting System uses HRP-conjugated secondary antibodies and ECL reagents for detection.
Supplier: Cytiva RPN2108
Includes: Anti-mouse HRP conjugate, 100μL; anti-rabbit HRP conjugate, 100μL; ECL Detection Reagents 1 and 2, 62.5mL each; Blocking agent, 5g
Description
- Provides rapid, sensitive results using simple protocols
- Designed for use with nitrocellulose and PVDF membranes
- Up to 10 times more sensitive than colorimetric methods
- Results obtained on X-ray film provide hard copy data and avoid fading associated with colorimetric detection methods
- Membranes can be reprobed up to 10 times
- Linear response in the range 0.2 to 2 O.D. units using pre-flashed Hyperfilm™ ECL
- Non-radioactive
Specifications
| Western Blot | |
| Ea. | |
| 100μL Anti-Mouse HRP conjugate, 100μL Anti-Rabbit HRP conjugate, 62.5mL ECL™ detection reagent 1, 62.5mL ECL™ detection reagent 2, 25g blocking agent, sufficient for 10 blots (10 x 10) |