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Invitrogen™ mMESSAGE mMACHINE™ SP6 Transcription Kit

Catalog No. AM1340
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25 Reactions
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AM1340 25 Reactions
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Catalog No. AM1340 Supplier Invitrogen™ Supplier No. AM1340
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Description

in vitro synthesis of large amounts of capped RNA

mMESSAGE mMACHINE™ kits are designed for in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end.

  • RNA Yield: 5-25μg
  • Include cap analog [m7G(5′)ppp(5′)G] in an ultra high-yield transcription reaction
  • Cap analog is incorporated only as first or 5′ terminal G of transcript because its structure precludes its incorporation at other position in RNA molecule
  • Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
  • Ideal for routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications

Specifications

Content And Storage SP6 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Final Product Type Synthetic mRNA, mRNA
Format Kit
Sample Type mRNA, Synthetic mRNA
For Use With (Application) In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions 25 Reactions
Product Line Ambion, mMESSAGE mMACHINE
Product Type SP6 In Vitro Transcription kit
Quantity 25 Reactions
Promoter SP6
Shipping Condition Dry Ice
Yield 15-25 μg
Starting Material PCR Products, Linearized DNA plasmid
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Frequently Asked Questions (FAQs)

What is the control included in the mMESSAGE mMACHINE kit?

The control is a pTRI-Xef control template which is a linearized TRIPLEscript plasmid contianing the 1.8 kb Xenopus elongation factor 1alpha gene under the transcription control of tandem SP6, T7, and T3 promoters (pTRI-Xef 1). Any one of the three polymerases can be used to synthesize the control RNA.

For Research Use Only. Not for use in diagnostic procedures.