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Cytiva AlkPhos Direct™ Hybridization Buffer for Direct Labeling System

Fast and convenient system for all high-sensitivity applications using DNA, RNA, or oligonucleotide probes

Manufacturer:  Cytiva RPN3688

Catalog No. 45-000-488

Includes: Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 5000cm2 membrane: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star™ or ECF detection reagents.



  • Hybridization stringency can be controlled by elevating temperatures up to 75°C (167°F), or by varying salt concentration
  • Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
  • ECF™ detection for quantitation experiments
  • Thirty minutes from completion of hybridization to application of detection reagent: saves two to three hours over conventional indirect methods
  • Resulting probes can be used without purification



For 5000 cm2 membrane
Amersham AlkPhos Direct Hybridization Buffer sufficient for 5000 cm2
Labeling reagent, crosslinker, control DNA, Reaction Buffer, hybridization Buffer, blocking agent, CDP-Star™ or ECF detection reagents
Fast, convenient for high-sensitivity applications with DNA, RNA, Oligo probes


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