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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual 
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
Supplier: Applied Biosystems™ 4387391M
Description
- Recommended for RNA pathogen amplification
- Consistent amplification of RNA targets with high specificity and sensitivity
- Optimized to work with your target-specific primers and probes
- Contains ROX for quantitative fluorescent signal normalization
AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.
Specifications
| 25X | |
| Works on all AB platforms, but is optimized for the 7500. | |
| Tube | |
| 1-step RT-qPCR | |
| Standard | |
| ROX (Pre-mixed) | |
| One-Step RT-PCR Reagent | |
| ArrayScript™ | |
| 25 X | |
| 14 mL |
| Sufficient for 1000 reactions of 25 μL, contains: • 14 mL 2 x RT-PCR Buffer • 1100 μL 25 x RT-PCR Enzyme Mix • 25 mL Nuclease-free Water. |
|
| Primer-probe | |
| High | |
| AmpliTaq Gold DNA Polymerase | |
| 7500 System | |
| AgPath-ID, Ambion | |
| 1000 Reactions | |
| 1000 Reactions | |
| 1000 Reactions |
For Research Use Only. Not for use in diagnostic procedures.