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  7. Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual DFS Item

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

$386.00 - $3050.00

Specifications

Concentration 25X
Description Works on all AB platforms, but is optimized for the 7500.
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
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Products 3
Catalog Number Mfr. No. No. of Reactions Price Quantity  
Catalog Number Mfr. No. No. of Reactions Price Quantity  
4387424M DFS Item
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Applied Biosystems™
4387424M 		500 Reactions
Each for $1,790.00
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4387391M DFS Item
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Applied Biosystems™
4387391M 		1000 Reactions
Each for $3,050.00
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AM1005M DFS Item
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Applied Biosystems™
AM1005M 		100 Reactions
Each for $386.00
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Description

Description

  • Recommended for RNA pathogen amplification
  • Consistent amplification of RNA targets with high specificity and sensitivity
  • Optimized to work with your target-specific primers and probes
  • Contains ROX for quantitative fluorescent signal normalization

AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.

Specifications

Specifications

25X
Primer-probe
High
AmpliTaq Gold DNA Polymerase
7500 System
AgPath-ID™, Ambion™
ArrayScript™
Works on all AB platforms, but is optimized for the 7500.
Tube
1-step RT-qPCR
Standard
ROX (Pre-mixed)
One-Step RT-PCR Reagent
25 X
Videos
SDS
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For Research Use Only. Not for use in diagnostic procedures.