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Applied Biosystems™ Absolute Q™ 1-step RT-dPCR Master Mix (4X)

Catalog No. A55146
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A55146 200 reactions
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Catalog No. A55146 Supplier Applied Biosystems™ Supplier No. A55146
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Description

Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps.

  • Optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps
  • The 4X formulation enables analysis of higher sample volumes and delivers accurate quantification of RNA targets without using a standard curve
  • Set up the reaction like 1-Step RT-qPCR, then load and run the plate in a single instrument
  • Requires just 5 minutes of hands-on time and RT-dPCR run time of ∼65 minutes
  • Ability to detect less than 1 copy/μL of a specific target within a 4-plex reaction
  • Detect up to four targets in a single reaction
  • Highly reproducible results between technical replicates, consistently generate >20,000 analyzable micro-chambers

Specifications

Detection Method Primer-probe
PCR Method Digital PCR
Polymerase Taq DNA Polymerase
Reaction Speed <2 hr.
Technique Digital PCR
Quantity 200 reactions
Compatibility Absolute Q Digital PCR System
Hot Start Hot Start Taq Polymerase
No. of Reactions 200 Reactions
Sample Type RNA
Volume 450 μL
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Frequently Asked Questions (FAQs)

Can I use TaqMan SNP Genotyping Assays to detect single-nucleotide variants (SNVs) that are present in less than 5% of a sample?

TaqMan SNP Genotyping Assays are designed to work on equal volumes, so if the mutation is only present at 5%, it is possible that the assay will not detect the variants. Therefore, we do not recommend using these assays to detect somatic mutations. SNP assays by qPCR with TaqMan SNP Genotyping Assays are intended to detect germline mutations.

For somatic mutations, we recommend using digital PCR or castPCR. Find more information about digital PCR and castPCR in the links below:

Digital PCR Assays and Kits

QuantStudio Absolute Q Digital PCR System

Absolute Q 1-Step RT-dPCR Master Mix (4X) (Cat. No. A55146) User Guide

TaqMan Mutation Detection Assay with castPCR Technology (Cat. Nos. 4465804, 4465807)

TaqMan Mutation Detection Assay: Competitive Allele-Specific TaqMan PCR

For research use only. Not for use in diagnostic procedures.