Flow Cytometry Compensation, Counting and Calibration
Invitrogen™ eBioscience™ IC Fixation Buffer
Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.
Invitrogen™ eBioscience™ Permeabilization Buffer (10X)
Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.
Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent
For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.
Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer
A buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative. Designed for use in immunofluorescent staining protocols of cells in suspension.
Invitrogen™ UltraComp eBeads™ Compensation Beads
Designed for use in compensation with all fluorochromes excited by ultraviolet, violet, blue, green, yellow-green, and red lasers. UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test.
Invitrogen™ 123count eBeads™ Counting Beads
Intended for use in absolute counting of cells or other particles by flow cytometry. 123count eBeads are 7μm microparticles containing encapsulated dyes compatible with blue (488nm) and violet (405nm) excitation sources and emitting fluorescence between approximately 500nm and 750nm.
Molecular Probes™ Flow Cytometry Size Calibration Kit (nonfluorescent microspheres)
Flow Cytometry Size Calibration Kit (nonfluorescent microspheres)
Invitrogen™ OneComp eBeads™ Compensation Beads
Spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls. OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test
Invitrogen™ UltraComp eBeads™ Spectral Unmixing Beads
UltraComp eBeads Spectral Unmixing Beads enhance spectral flow cytometry by providing precise single-color spectral unmixing control. Compatible with various antibodies and laser excitations, these third-generation beads offer optimized fluorophore unmixing, ensuring accurate results and superior performance.
Invitrogen™ eBioscience™ Mouse Regulatory T Cell Staining Kit #2
Formulated & optimized for the staining of mouse splenocytes with the FJK-16s monoclonal antibody. eBioscience™ Mouse Regulatory T Cell Staining Kit #2 includes: Anti-Mouse CD4 FITC, CD25 PE and CD16/32, Anti-Mouse/Rat Foxp3 APC, Rat IgG2a Isotype Control APC, staining buffer, fixation concentrate.
Invitrogen™ eBioscience™ Essential Human Treg Phenotyping Panel & Staining Buffers
Invitrogen™ eBioscience™ Essential Human Treg Phenotyping Panel and Staining Buffers are designed for flow cytometry-based identity testing of regulatory T-cells (Tregs).
Invitrogen™ Cell Sorting Set-up Beads (for blue lasers)
Approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell sorting system, including laser source, optics, and stream flow.
Invitrogen™ eBioscience™ Human Regulatory T Cell Staining Kit #2
Contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.