Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (4,081)

Singleplex and Multiplex Bead Based Immunoassays (526)

ELISA Substrates and Reagents (83)

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Invitrogen™ Human IL-6 Uncoated ELISA Kit

ELISA

Pricing and Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	Il6
Total Assay Time	24 hr 30 min
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2-8°C
Assay Range	2-200 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	P05231
Regulatory Status	RUO
Target	IL-6
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X), Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X),Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3569
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Interleukin-6

Invitrogen™ Human VEGF ELISA Kit

ELISA

Pricing and Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	8.1%
Intraassay CV	4.7%
Incubation Time	4 hr.
Gene Symbol	VEGFA
Total Assay Time	4 hr
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	<5 pg/mL
Storage Requirements	2-8°C
Assay Range	23.4-1500 pg/mL
Marker Gene	VEGF
For Use With (Application)	Colorimetric Detection
Test Time	1 hr 20 min
Accession Number	P15692
Regulatory Status	RUO
Target	VEGF
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7422
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Mouse IL-5 Uncoated ELISA Kit

ELISA

Pricing and Availability
Specifications

Immunoassay Kit Format	Uncoated ELISA Kit
Content And Storage	Capture Antibody: Pre-titrated, purified antibody Detection Antibody: Pre-titrated, biotin-conjugated antibody Standard: Recombinant protein for generating standard curve and calibrating samples 10 x Coating Buffer: Buffer for plating the Capture Antibody 5 x ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme Enzyme: Pre-titrated Avidin-HRP Substrate: 1 x TMB Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96-well Plate: Corning Costar 9018 (included with product Cat. No. ending in suffixes -22, -76, -86)
Target Species	Mouse
Conjugate	HRP
Gene Symbol	Il5
Total Assay Time	24 hr 30 min
Sample Volume	Lavage Fluid 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	8 pg/mL
Storage Requirements	2-8°C
Assay Range	8-500 pg/mL
Test Time	1 hr 45 min
Accession Number	P04401
Regulatory Status	RUO
Target	IL-5
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Gene Alias	B-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; IL5; IL-5; ILN; Interleukin; Interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRFB-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; IL5; IL-5; ILN; Interleukin; Interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRF; CANDF6; IL-17F; ML-1; ML1
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16191
Sample Type	Lavage Fluid,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human PF4 ELISA Kit

ELISA

Pricing and Availability
Specifications

Protein Family	Chemokines & Receptors
Target Species	Human
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Assay Sensitivity	20 pg/mL
Research Discipline	Inflammation
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	PF4
Specificity	This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Interassay CV	<12%
Gene Symbol	Pf4
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	20.58-15000 pg/mL
Accession Number	P02776
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	5196
Sample Type	Plasma,Serum,Supernatant
Synonym	CXCL4, PF-4, SCYB4

Invitrogen™ Human Cathepsin D ELISA Kit

ELISA

Pricing and Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	Ctsd
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.049 ng/mL
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	0.049-12 ng/mL
Test Time	1 hr 20 min
Accession Number	P07339
Regulatory Status	RUO
Target	Cathepsin D
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	1509
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Serotonin Competitive ELISA Kit

ELISA

Pricing and Availability
Specifications

Shipping Condition	Wet or Dry Ice
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	All Species
Conjugate	HRP
Interassay CV	<10%
Intraassay CV	<10%
Total Assay Time	2 hr 30 min
Assay Sensitivity	9.38 ng/mL
Storage Requirements	Refer to product documentation for component specific storage temperature.
Assay Range	15.63-1000 ng/mL
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	Serotonin
Kit Contents	Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,Biotinylated Detection Antibody,HRP Conjugate,Standard & Sample Diluent,Biotinylated Detection Antibody Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer
Gene Alias	5-HT; 5-hydroxytryptamne5-HT; 5-hydroxytryptamne;
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Sample Type	Plasma,Serum
Label or Dye	Biotin

Invitrogen™ Cell Extraction Buffer

Suitable for use in ELISA and Western blotting

Pricing and Availability