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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (3,999)
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1

Invitrogen™ SARS-CoV-2 Neutralizing Ab ELISA Kit  DFS Item

ELISA

Shipping Condition Wet Ice
Content And Storage 2°C to 8°C
Host Species Human
Conjugate HRP
Interassay CV <10%
Intraassay CV <10
Total Assay Time 1 hrs 30 mins
Sample Volume Plasma 5 μL, Serum 5 μL
Protein SARS-CoV-2 Spike Protein RBD
Test Time 40 mins
Regulatory Status RUO
Target SARS-CoV-2 Spike Protein RBD
Kit Contents Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum
2

Invitrogen™ Human IL-2 Uncoated ELISA Kit with Plates

ELISA

Target Species Human
Conjugate HRP
Gene Symbol Ctla-8,Ctla8,IL-17,IL-17 A,Il17
Total Assay Time 24 hrs 30 mins
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity 2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 2 to 250 pg/mL
For Use With (Application) ELISA
Test Time 1 hrs 45 mins
Accession Number P60568
Regulatory Status RUO
Target IL-2
Kit Contents Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3558
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Interleukin-2
3

Invitrogen™ Human IL-2 Uncoated ELISA Kit

ELISA

Target Species Human
Conjugate HRP
Gene Symbol Il-1 A
Total Assay Time 24 hrs 30 mins
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity 2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 2 to 250 pg/mL
For Use With (Application) ELISA
Test Time 1 hrs 45 mins
Accession Number P60568
Regulatory Status RUO
Target IL-2
Kit Contents Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3558
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Interleukin-2
5

Invitrogen™ Human IL-12 p70 Uncoated ELISA Kit

ELISA

Target Species Human
Conjugate HRP
Gene Symbol IFN-g,Ifg
Total Assay Time 24 hrs 30 mins
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity 4 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 4 to 500 pg/mL
For Use With (Application) ELISA
Test Time 1 hrs 45 mins
Accession Number P29459,P29460
Regulatory Status RUO
Target IL-12 p70
Kit Contents Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Streptavidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3592, 3593
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Interleukin-12, IL12, IL23
6

Invitrogen™ Monkey IGFBP-3 ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 1.02 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 1.024 to 250 ng/mL
Test Time 1 hrs 20 mins
Accession Number acid stable subunit of the 140 K IGF complex; AI649005; binding protein 29; binding protein 53; BP-53; growth hormone-dependent binding protein; I79
Regulatory Status RUO
Target IGFBP3
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
7

Invitrogen™ Rat IgG2a Uncoated ELISA Kit

ELISA

Target Species Rat
Conjugate HRP
Total Assay Time 24 hrs 30 mins
Sample Volume Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity 0.39 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.39 to 25 ng/mL
For Use With (Application) ELISA
Test Time 1 hrs 45 mins
Regulatory Status RUO
Target Rat IgG2a
Kit Contents Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Rat IgG2a protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum,Supernatant
Synonym Immunoglobulin G2a
8

Invitrogen™ Human S100 A11 ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol S100A11
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.03 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.034 to 25 ng/mL
Test Time 1 hrs 20 mins
Accession Number cal; Calgizzarin; calizzarin; EMAP; Emap1; EMAPI; endothelial monocyte activating polypeptide 1; endothelial monocyte-activating polypeptide; epididymis secretory protein Li 43; HEL-S-43; Metastatic lymph node gene 70 protein; MLN 70; MLN70; Protein S100-A11; Protein S100-A11, N-terminally processed; Protein S100C; Protein S100-C; S100 calcium binding protein A11; S100 calcium binding protein A11 (calgizzarin); S100 calcium binding protein A11 (calizzarin); S100 calcium binding protein A14; S100 calcium-binding protein A11; S100 calcium-binding protein A11 (calgizzarin); S100A11; S100a14; S100C
Regulatory Status RUO
Target S100A11
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 6282
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
9

Invitrogen™ Human FGF4 ELISA Kit  DFS Item

ELISA

Protein Family Growth Factors & Receptors
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 50 pg/mL
Research Discipline Apoptosis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target FGF4
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-6, FGF-7, FGF-9, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Interassay CV <12%
Gene Symbol HBGF-4,HST,HST-1,HSTF1,K-FGF,KFGF
Storage Requirements 2°C to 8°C
Assay Range 24.69 to 18,000 pg/mL
Accession Number P08620
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 2249
Sample Type Plasma,Serum,Supernatant
Synonym HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF
10

Invitrogen™ Human Furin ELISA Kit  DFS Item

ELISA

Protein Family Growth Factors & Receptors
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 15 pg/mL
Research Discipline Neurobiology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target Furin
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Interassay CV <12%
Gene Symbol FUR,PACE,PCSK3,SPC1
Storage Requirements 2°C to 8°C
Assay Range 13.72 to 10,000 pg/mL
Accession Number P09958
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 5045
Sample Type Plasma,Serum,Supernatant
Synonym FUR, PACE, PCSK3, SPC1
11

Invitrogen™ Human ANGPTL4 ELISA Kit  DFS Item

ELISA

Protein Family Other Proteins
Target Species Human
Conjugate HRP
Interassay CV <12%
Gene Symbol ARP4,FIAF,HARP,HFARP,NL2,PGAR,TGQTL,UNQ171,pp1158
Sample Volume Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Assay Sensitivity 20 pg/mL
Storage Requirements 2°C to 8°C
Research Discipline Angiogenesis
Assay Range 27.4 to 20,000 pg/mL
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Assay Solid-phase sandwich ELISA
Accession Number Q9BY76
Regulatory Status RUO
Target ANGPTL4
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 51129
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym PP1158, ANGPTL2, ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158
13

Invitrogen™ Human TNF alpha (Total) ELISA Kit

ELISA

Target Species Human
Conjugate HRP
Interassay CV 8.1%
Intraassay CV 7.7
Gene Symbol DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF
Total Assay Time 3 hrs 10 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 5.0 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 23 to 1,500 pg/mL
For Use With (Application) ELISA
Test Time 1 hrs 20 mins
Accession Number P01375
Regulatory Status RUO
Target TNF alpha
Kit Contents Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 7124
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Cachectin, Tumor necrosis factor alpha, DIF
14
Promotions Available

Invitrogen™ Human CFHR1 ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol CFHR1
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.14 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.14 to 100 ng/mL
Test Time 1 hrs 20 mins
Accession Number AI194696; CFHL; CFHL1; CFHL1P; CFHR1; CFHR1P; CFHRB; complement component factor h-like 1; complement factor H related 1; complement factor H-related 1; complement factor H-related 1 pseudogene; complement factor H-related protein; complement factor H-related protein 1; FHR1; FHR-1; H factor (complement)-like 1; H factor (complement)-like 2; H factor-like protein 1; H36; H36-1; H36-2; H-factor-like 1; HFL1; HFL2
Regulatory Status RUO
Target CFHR1
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3078
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
15
Promotions Available

Invitrogen™ Porcine IFN-gamma ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol IFNG
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.5 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.5 to 150 ng/mL
Test Time 1 hrs 20 mins
Accession Number BoIFNG; cytokine; EGK
Regulatory Status RUO
Target IFN gamma
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 396991
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP