Reverse Transcriptases
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Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL)
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.
| Température de réaction optimale | 37°C |
|---|---|
| Type de réactif | Reverse Transcription |
| Vitesse de réaction | 50 min. |
| Format | Stand-alone Enzyme |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Yes |
| Technique | Reverse Transcription |
| Concentration | 200 U/μL |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 7 kb |
| Conditions d’expédition | Wet Ice |
| Transcriptase inverse | M-MLV |
Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.
| Vitesse de réaction | 60 min. |
|---|---|
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 200 U/μL |
| Conditions d’expédition | Dry Ice |
| Contenu et stockage | • RevertAid H Minus Reverse Transcriptase (200 U/μL) |
| Température de réaction optimale | 42°C to 45°C |
| Type de réactif | Reverse Transcription |
| Format | Stand-alone Enzyme |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 13 kb |
| Transcriptase inverse | RevertAid H Minus |
Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
| Vitesse de réaction | 60 min. |
|---|---|
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Yes |
| Concentration | 200 U/μL |
| Conditions d’expédition | Dry Ice |
| Contenu et stockage | • RevertAid Reverse Transcriptase (200 U/μL) |
| Température de réaction optimale | 42°C |
| Type de réactif | Reverse Transcription |
| Format | Stand-alone Enzyme |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 13 kb |
| Transcriptase inverse | RevertAid |
Invitrogen™ MultiScribe™ Reverse Transcriptase
MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.
Thermo Scientific™ Maxima Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.
| Concentration | 200 U/μL |
|---|
Invitrogen™ SuperScript™ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | 50 min. |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 200 U/μL |
| Conditions d’expédition | Wet Ice |
| Température de réaction optimale | 42°C |
| Type de réactif | Reverse Transcription |
| Format | Stand-alone Enzyme |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 12.3 kb |
| Transcriptase inverse | SuperScript II |
Invitrogen™ SuperScript™ IV Reverse Transcriptase
SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | 10 min. |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 200 U/μL |
| Conditions d’expédition | Dry Ice |
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Format | Stand-alone Enzyme |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 12.3 kb |
| Transcriptase inverse | SuperScript IV |
Invitrogen™ SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | 30 to 50 min. |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 200 U/μL |
| Conditions d’expédition | Dry Ice |
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Format | Stand-alone Enzyme |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 12.3 kb |
| Transcriptase inverse | SuperScript III |
Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | 15 to 30 min. |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 200 U/μL |
| Conditions d’expédition | Dry Ice |
| Température de réaction optimale | 50°C to 55°C |
| Type de réactif | Reverse Transcription |
| Format | Stand-alone Enzyme |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 20 kb |
| Transcriptase inverse | Maxima H Minus |
G-Biosciences Reverse Transcriptase (RT)
Reverse Transcriptase is used for first strand cDNA synthesis and gene expression analysis.
| GC-Rich PCR Performance | Medium |
|---|---|
| Méthode de détection | qPCR |
| Vitesse de réaction | Fast or Standard |
| Type de produit | Reverse Transcriptase |
| Méthode PCR | qPCR,Multiplex qPCR,1-step RT-qPCR,2-step RT-qPCR,1-step RT-PCR |
| À utiliser avec (application) | Gene Expression,Microbial Detection,Pathogen Detection,Virus Detection,cDNA Synthesis |
| Marqueur ou colorant | Biotin,FAM,SYBR Green,ROX,TAMRA |
| Forme | Liquid |