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0.05% Trypsin and 0.5mM EDTA in modified HBSS w/o Ca++ and Mg++, 100 mL
Typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
A column-based approach to antibody biotinylation and clean-up