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MP Biomedicals™ Trypsin, Porcine Pancreas
Typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
Supplier: MP Biomedicals™ 0215357125
Description
- CAS Number: 9002-07-7
- Molecular Weight: Approximately 23,000 or 22000 (for human)
- Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
- Activity: One BAEE unit will produce a DA253 of 0.001 per minute at pH 7.6 at 25°C using BAEE as substrate. Reaction volume = 3.2mL (1 cm light path).
One TAME unit hydrolyzes 1 umole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 0.001 M calcium ion.
One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified. - Activity Conversion:1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units
- Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
Specifications
| Release adherent cells from tissue culture plates for passaging | |
| 4°C | |
| 25 g |
| Porcine | |
| Powder | |
| Trypsin |