Gel Electrophoresis Buffers and Diluents
Buffer and diluent solutions to prepare, dilute, and deliver protein and nucleic acid samples to electrophoresis gels for separation and analysis; includes solutions to stabilize, control the concentration of, denature, and reduce sample proteins.
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Thermo Scientific Chemicals Phosphate, 0.5M buffer soln., pH 8.0
CAS: 7558-80-7 Molecular Weight (g/mol): 119.976
| CAS | 7558-80-7 |
|---|---|
| Molecular Weight (g/mol) | 119.976 |
| CAS | 127-09-3 |
|---|
Thermo Scientific Chemicals Phosphate, 0.2M buffer soln., pH 7.0
CAS: 7558-80-7 Molecular Weight (g/mol): 119.976
| CAS | 7558-80-7 |
|---|---|
| Molecular Weight (g/mol) | 119.976 |
Invitrogen™ NativePAGE™ 5% G-250 Sample Additive
Add to samples prepared with the NativePAGE Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE gels
| Packaging | Plastic Bottle |
|---|---|
| Physical Form | Liquid |
| Recommended Storage | Ambient Temperatures |
| Packaging | Plastic Bottle |
|---|---|
| Physical Form | Liquid |
| Recommended Storage | Ambient Temperatures |
Invitrogen™ 4X Bolt™ LDS Sample Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
| Buffer | Sample Loading Buffers |
|---|---|
| Content And Storage | Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red. Store at 4°C to 25°C. |
| Gel Type | Bis-Tris Plus |
| For Use With (Equipment) | Bolt Mini Gel Tank |
| Product Type | LDS Sample Buffer |
| Concentration | 4X |
| Gel Compatibility | Bolt™ Bis-Tris Plus Gels |
| Product Line | Bolt |
Invitrogen™ Digitonin (5%)
Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.
Invitrogen™ Gel Loading Buffer II (Denaturing PAGE)
A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store at –20°C. |
| Format | Bottle |
| Form | Liquid |
| Gel Compatibility | Denaturing Polyacrylamide Gels |
| Product Line | Ambion |