Gel Electrophoresis Buffers and Diluents
FRAP1 (mTOR) Assay Buffer
FRAP1 (mTOR) Assay Buffer
Thermo Scientific™ Pierce™ 10X Western Blot Transfer Buffer, Methanol-free
A methanol-free concentrated solution to easily and quickly prepare MeOH-free Western blot transfer buffer for tank-based or other wet gel-to-membrane transfer methods.
MilliporeSigma™ Novagen™ Ni-NTA Buffer Kit
Used for purification of HisTag fusion proteins on Ni-NTA HisBind Resin.
MilliporeSigma™ Beclin-1 Activator I, TAT-Beclin-1, Calbiochem™,
A cell-permeable Atg6/Beclin1-derived autophagy-inducing peptide, Peptide Sequence: H-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-Gly-Thr-Asn-Val-Phe-Asn-Ala-Thr-Phe-Glu-Ile-Trp-His-Asp-Gly-Glu-Phe-Gly-Thr-OH; Packaged under inert gas
MilliporeSigma™ Phenol:Chloroform:Isoamyl Alcohol, 25:24:1, OmniPur™, Calbiochem™,
TE Buffered Saturated pH 6.7/8.0; DNA applications
Corning™ 3D Clear Tissue Clearing Reagent
Corning™'s 3D Clear tissue clearing technique is designed specifically to support imaging for 3D cell culture models and plate-based high-throughput processing. When paired with fluorescent labeling (e.g. fluorescent protein, immunofluorescence, chemical dyes) and high content confocal microscopy, Corning™'s 3D Clear Reagent allows for complete 3D cell culture model characterization and more accurate drug screening.
MilliporeSigma™ Novagen™ 4X SDS Sample Buffer
Used for SDS-PAGE analysis of proteins.
Corning™ 3D Clear Penetration Buffer
Corning™'s 3D Clear Penetration Buffer is used with the Corning™ 3D immunolabeling process and is comprised of PBS/0.2% Triton™ X-100/0.3 M glycine/20% DMSO.
MilliporeSigma™ Imidazole, ULTROL™ Grade, Calbiochem™,
Useful in the pH range of 6.2 to 7.8
MilliporeSigma™ PIPPS, Calbiochem™,
A non-metal complexing, zwitterionic buffer with mixed-mode dissociation constants of pKa1 = 3.73 and pKa2 = 7.96 (100mM, 25°C)
Corning™ 3D Clear Washing Buffer 10X
Corning™'s 3D Clear Washing Buffer 10X is used for the Corning™ 3D Clear immunolabeling process. This solution is a concentrate and is comprised of: PPBS with 0.2% Tween-20 and 10 μg/mL heparin. This solution should be diluted to 1X concentration with PBS prior to use.
MilliporeSigma™ Novagen™ PhosphoSafe™ Extraction Reagent
Extracts cytosolic proteins from mammalian insect cells while preserving phosphorylation state.
