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  6. Invitrogen™ Novex™ Tris-Glycine Mini Protein Gels, 10%, 1.0 mm, WedgeWell™ format

Invitrogen™ Novex™ Tris-Glycine Mini Protein Gels, 10%, 1.0 mm, WedgeWell™ format

Catalog No. XP00100PK2
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Catalog No. Wells Quantity
XP00100PK2 10-well 2 gels (1 box)
XP00100BOX 10-well 10 gels (1 box)
XP00102BOX 12-well 10 gels (1 box)
XP00105BOX 15-well 10 gels (1 box)
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Description

Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity.

Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity. Novex Tris-Glycine Mini Gels are based on traditional Laemmli protein electrophoresis that allow the use of Laemmli sample and running buffers.

Features of Novex Tris-Glycine gels, WedgeWell format, include:
• Improved shelf life—store gels for up to 8 months at 4°C
• Diversity—use for native and denaturing protein assays
• Wedge-shaped wells—easily load up to two times more sample volume
• Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.

Run your proteins in native or denatured form
Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC26755). For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer (LC2673) and Novex Tris-Glycine Native Running Buffer (LC2672).

Convenient to use
Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer (LC3675) when preforming traditional wet transfer using the Invitrogen Mini Blot Module (B1000). Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).

Specifications

Gel Percentage 10%
Gel Size Mini
Gel Thickness 1.0 mm
Gel Type Tris-Glycine Plus
Recommended Applications Denaturing, Native
Sample Loading Volume Up to 60 μL
Separation Range 30 to 250 kDa
Separation Type Denaturing, Native
Wells 10-well
Mode of Separation Molecular Weight
Quantity 2 gels (1 box)
Shipping Condition Wet Ice
Storage Requirements Store at 2°C to 8°C. Do not freeze.
Length (Metric) 8 cm
Width (Metric) 8 cm
Product Line Novex, WedgeWell
For Use With (Equipment) Mini Gel Tank
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Frequently Asked Questions (FAQs)

Is it okay to use protein gels past their expiration date?

We do not recommend using gels past their expiration date because over time, the polyacrylamide hydrolyzes to acrylic acid and ammonia and this will affect the resolution of the proteins. Breakdown of polyacrylamide matrix is identified by:
- Ghost bands and doublets, seen first in the high molecular weight proteins
- Smiling of dye front across the gel, with bands in outer lanes becoming very slanted - proteins run slower there due to change in pH and pore size over time.
Can I run your protein gels overnight?

This is not really recommended, but it is always possible to increase run time by lowering the voltage of the run. In general, the relationships are linear - i.e., decreasing voltage by half will double the run time.
Do I need to increase the voltage when I run a 1.5 mm protein gel versus a 1.0 mm gel?

If you are running the gel at constant voltage, you do not need to increase the voltage regardless of the thickness of the gel.

Why do you recommend running your protein gels at constant voltage?

Using constant voltage allows the current and power to decrease during the run, providing a safety margin in case of a break in the system. Having lower power is a safety benefit and will also decrease the chances of experiencing an overheating of the gel. Further, the constant voltage setting does not need adjustment to account for differences in number or thickness of gels being run.

Can Novex WedgeWell gels be stored at room temperature?

No. Novex WedgeWell gels must be stored at 4 degrees C.
Do the Novex WedgeWell gels require any specific buffers?

The Novex WedgeWell gels can use standard Tris-Glycine sample and running buffers. For running under native conditions, we recommend using sample and running buffers that do not contain SDS (such as our Native Tris-Glycine premade buffers).
With Novex WedgeWell gels, what in the chemistry changed to allow for a longer shelf life?

We were able to optimize shelf life through a proprietary gel formulation change. Unfortunately, we are unable to provide specific details on the chemical changes.
What is the orientation of the printing on the cassette for the Novex WedgeWell gels?

We use what we call ‘mirror printing', the same way we do for Bolt gels. This means that when the gel is loaded in the Mini Gel Tank, the text will face in the readable direction (left to right).
Can the new Novex WedgeWell gels be run in the XCell SureLock Mini-Cell?

Yes, the new Novex WedgeWell gels can be run in the XCell SureLock Mini-Cell.
I am transferring a Tris-Glycine gel using constant voltage and the current reading is way over the expected starting current. Can you offer some suggestions?

The most common cause of abnormally high current is the transfer buffer. If the transfer buffer is too concentrated, this leads to increased conductivity and current. High current may also occur if Tris-HCl is accidentally substituted for the Tris base required in the transfer buffer. This will again result in low buffer pH and lead to increased conductivity and current and subsequently, overheating. We recommend checking the transfer buffer and its reagent components and re-diluting or remaking the buffer.