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TSKgel™ Phenyl-5PW RP HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Catalog No. 111021803
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Length (Metric):
7.5 cm
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Catalog No. Length (Metric)
111021803 7.5 cm
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Catalog No. 111021803 Supplier Merck Emd Millipore Supplier No. SGMA808043
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Description

TSKgel™ Phenyl-5PW RP columns are filled with 10 micron porous polymethacrylate particles, the surface of which is covered with a high density of phenyl groups that causes proteins to be retained in a low salt buffer mobile phase and requiring a solvent gradient for their elution, as is the protocol in reversed phase chromatography.

In contrast, TSKgel™ Phenyl-5PW has such a low density of phenyl groups on the surface that proteins can only be retained at high salt concentration and eluted by a decreasing salt gradient, which, of course, is the protocol used in hydrophobic interaction chromatography. TSKgel™ Phenyl-5PW RP columns can withstand rigorous cleaning with either acid or base. The 1000 Å pore size accommodates globular protein samples up to 1,000,000 Da.

Order Info

Shipped in 30% methanol - 70% water.

Specifications

Diameter (Metric) 4.6 mm
Flow Rate 1.2 mL/min.
Length (Metric) 7.5 cm
Ligand Type 1 meq/mL ligand density (estimate)
Max. Pressure 30 bar
Particle Size 10 μm
pH 2.0 - 12
Pore Size 1000 Å
Solvents 0 to 100% organic solvent
Temperature 4°C to 60°C
Stationary Phase Phenyl
Product Line TSKgel
Matrix Polymer Particle Platform
Phase Reversed Phase
Material Stainless Steel
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TSKgel is a registered trademark of Tosoh Corporation