TSKgel™ NH2-100 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ NH2-100 columns are unique in that the bonded phase ligand not only, as expected, has a terminal primary amino group, but that the spacer also incorporates secondary as well as tertiary amino groups. Anionic compounds are retained on the column by ionic interaction. This allows for the use of gradients in salt concentration, in addition to gradient elutions with acetonitrile. Since the TSKgel™ NH2-100 columns have cationic sites, the columns can be used as mixed mode columns under some conditions. TSKgel™ NH2-100 columns are well suited for the analysis of all types of hydrophilic compounds, including carbohydrates and peptides. Due to a high ligand density and large surface area, these columns show stronger retention of polar compounds than TSKgel™ Amide-80 columns.