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  6. TSKgel™ Chelate-5PW HPLC Column, Sold by MilliporeSigma™ Supelco™

TSKgel™ Chelate-5PW HPLC Column, Sold by MilliporeSigma™ Supelco™

Catalog No. 111021849
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Diameter (Metric):
5 mm
7.5 mm
21.5 mm
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Catalog No. Diameter (Metric)
111021849 5 mm
111021816 7.5 mm
111021817 21.5 mm
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Catalog No. 111021849 Supplier Merck Emd Millipore Supplier No. 814440
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Description

Utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, loaded with divalent metal ions by chelation.

  • High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins
  • Utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+
  • Column is loaded with divalent metal ions by chelation
  • Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at a neutral pH
  • Retained compounds can be eluted with buffer containing imidazole or glycine
  • A decreasing pH gradient can be used for elution purposes as well
  • For successful use of this retention mechanism, select the proper metal ions for chelation and an elution buffer to desorb the analytes
  • Applications: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides

Specifications

Diameter (Metric) 5 mm
Flow Rate 1 mL/min
Length (Metric) 5 cm
Max. Pressure 20 bar
Particle Size 10 μm
pH 2 to 12
Solvents ≤ 20% Organic Solvent
Temperature ≤45°C
Stationary Phase Iminodiacetic Acid
Product Line TSKgel
Matrix Iminodiacetic Acid Phase/Hydroxylated Methacrylic Polymer
Material Glass
Technique Affinity
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TSKgel is a registered trademark of Tosoh Corporation