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TSKgel™ Boronate-5PW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™
Description
- High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins
- Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions
- This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds, and catecholamines
- Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers
- Adsorption onto the matrix takes place in basic buffers such as HEPES and morpholine
- Desorption takes place in carbohydrate or amine-containing mobile phases such as sorbitol or Tris
- Resin Adsorption Capacity: 40 μmol/mL
Specifications
Specifications
| Diameter (Metric) | 5 mm |
| Flow Rate | 1.2 mL/min |
| Length (Metric) | 5 cm |
| Max. Pressure | 30 bar |
| Particle Size | 10 μm |
| pH | 2 to 9 |
| Solvents | ≤ 20% Organic Solvent |
| Temperature | >0°C |
| Stationary Phase | M-Aminophenyl Boronate |
| Product Line | TSKgel |
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TSKgel is a registered trademark of Tosoh Corporation