Applied Biosystems™ TrueMark™ Urinary III Plus Combo Kit 

Description

The Applied Biosystems TrueMark Urinary III Plus Combo Kit achieves molecular detection through real-time PCR technology, offering an end-to-end workflow for many common infectious disease areas by utilizing the power of QuantStudio real-time PCR systems. The combination of a TrueMark panel (included) and a QuantStudio system helps enable rapid, accurate detection and categorization of microorganisms that cause urinary diseases.

The TrueMark Urinary III Plus panel is composed of convenient, duplexed TaqMan assays that detect Citrobacter freundii, Enterobacter cloacae, Enterococcus faecium, Klebsiella oxytoca, Serratia marcescens, Mycoplasma hominis, Morganella morganii, Providencia stuartii, and an internal control in the same reaction, utilizing real-time PCR techniques.

Features of the TrueMark Urinary III Plus panel include:
• Ease-of-use—convenient, duplexed reactions include assay and positive control, pre-spotted and dried down on TaqMan plates, designed for easy setup and increased accuracy; just add sample and TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX) (included)
• Endogenous controls—duplex reactions offer RNase P human internal control in every well for added confidence
• Turn-around time—choose from a selection of predefined panels with a short turn-around time
• Amplification control—TrueMark Amplification Control I covers all targets in RTI, UTI, STI, and vaginal panels
• Maximize the workflow—expand from SARS-CoV-2 testing to TrueMark infectious disease research panels utilizing the same workflow

The TrueMark Urinary III Plus Combo Kit includes the following components:
• TrueMark Urinary III Plus Panel—collection of duplex assays that contain primer/probe sets specific to Citrobacter freundii, Enterobacter cloacae, Enterococcus faecium, Klebsiella oxytoca, Serratia marcescens, Mycoplasma hominis, Morganella morganii, Providencia stuartii, and RNase P genomic regions
• TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX)—ready-to-use PCR mix, including reverse transcriptase, polymerase, dNTPs, salt, and buffer

The TrueMark Urinary III Plus Combo Kit is used with nasopharyngeal swab or nasopharyngeal aspirate samples on our QuantStudio real-time PCR systems.

Product use
The TrueMark Urinary III Plus Panel is a collection of duplex assays that detect microbes and an internal control in the same reaction for qualitative detection and identification of Citrobacter freundii, Enterobacter cloacae, Enterococcus faecium, Klebsiella oxytoca, Serratia marcescens, Mycoplasma hominis, Morganella morganii, Providencia stuartii in urine samples obtained from individuals exhibiting signs and symptoms of a urinary tract infection.