Learn More
Applied Biosystems™ TrueMark™ Urinary II Plus Combo Kit
Description
The Applied Biosystems TrueMark Urinary II Plus Combo Kit achieves molecular detection through real-time PCR technology, offering an end-to-end workflow for many common infectious disease areas by utilizing the power of QuantStudio real-time PCR systems. The combination of a TrueMark panel (included) and a QuantStudio system helps enable rapid, accurate detection and categorization of microorganisms that cause urinary diseases.
The TrueMark Urinary II Plus panel is composed of convenient, duplexed TaqMan assays that detect Corynebacterium riegelii, Enterobacter aerogenes, Aerococcus urinae, Proteus vulgaris, Acinetobacter baumannii, Actinobaculum schaalii, Enterococcus faecalis, Streptococcus agalactiae, and an internal control in the same reaction, utilizing real-time PCR techniques.
Features of the TrueMark Urinary II Plus panel include:
• Ease-of-use—convenient, duplexed reactions include assay and positive control, pre-spotted and dried down on TaqMan plates, designed for easy setup and increased accuracy; just add sample and TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX) (included)
• Endogenous controls—duplex reactions offer RNase P human internal control in every well for added confidence
• Turn-around time—choose from a selection of predefined panels with a short turn-around time
• Amplification control—TrueMark Amplification Control I covers all targets in RTI, UTI, STI, and vaginal panels
• Maximize the workflow—expand from SARS-CoV-2 testing to TrueMark infectious disease research panels utilizing the same workflow
The TrueMark Urinary II Plus Combo Kit includes the following components:
• TrueMark Urinary II Plus Panel—collection of duplex assays that contain primer/probe sets specific to Corynebacterium riegelii, Enterobacter aerogenes, Aerococcus urinae, Proteus vulgaris, Acinetobacter baumannii, Actinobaculum schaalii, Enterococcus faecalis, Streptococcus agalactiae, and RNase P genomic regions
• TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX)—ready-to-use PCR mix, including reverse transcriptase, polymerase, dNTPs, salt, and buffer
The TrueMark Urinary II Plus Combo Kit is used with nasopharyngeal swab or nasopharyngeal aspirate samples on our QuantStudio real-time PCR systems.
Product use
The TrueMark Urinary II Plus Panel is a collection of duplex assays that detect microbes and an internal control in the same reaction for qualitative detection and identification of Corynebacterium riegelii, Enterobacter aerogenes, Aerococcus urinae, Proteus vulgaris, Acinetobacter baumannii, Actinobaculum schaalii, Enterococcus faecalis, Streptococcus agalactiae in urine samples obtained from individuals exhibiting signs and symptoms of a urinary tract infection.
Specifications
Specifications
| Content And Storage | 5 plates; 3 x 1 mL Store panels at 15–30°C. Store master mix at 5 to -30°C. |
| Detection Method | Primer-probe |
| Label or Dye | 2 Color |
| Quantity | 1 kit |
| Target | Corynebacterium riegelii, Enterobacter aerogenes, Aerococcus urinae, Proteus vulgaris, Acinetobacter baumannii, Actinobaculum schaalii, Enterococcus faecalis, and Streptococcus agalactiae |
| For Use With (Application) | Pathogen Detection |
| Compatibility | QuantStudio™ 5 Real‐Time PCR System, 96-well, 0.2-mL block; QuantStudio™ 5 Dx Real‐Time PCR System, 96-well, 0.2-mL block; QuantStudio™ 7 Pro Real-Time PCR System, 96-well, 0.2-mL block |
| No. of Reactions | Up to 12 Samples/Plate |
Frequently Asked Questions (FAQs)
When adopting a new testing method for your research needs, we can assist in enabling the verification of the workflow to help ensure a successful launch. Our analytical validation (AV) consulting services can speed up the launch process by up to 75% and cut costs by up to half. Our expert team will consult to help develop and optimize your research workflow, while providing control material, data analysis support, and template documentation to fully maximize your instrument and reagent investment. Please refer to https://www.thermofisher.com/av or contact your sales representative for more information.
The recommended specimens that can be used with the TrueMark Infectious Disease panels are listed below:
- TrueMark Respiratory I Plus Combo Kit, TrueMark Respiratory II Plus Combo Kit, and TrueMark Respiratory III Plus Combo Kit: Nasopharyngeal (NP) or nasopharyngeal aspirate swabs
- TrueMark Urinary I Plus Combo Kit, TrueMark Urinary II Plus Combo Kit, and TrueMark Urinary III Plus Combo Kit: Urine samples
- TrueMark STI Plus Combo Kit: Vaginal and genital swabs
- TrueMark Vaginal Plus Combo Kit: Vaginal swabs
- TrueMark Lesion Plus Combo Kit: Lesion/genital swab
- TrueMark Genital Plus Combo Kit: Genital swab
Here are the controls listed in the TrueMark Infectious Disease panels user guide and their considerations for use:
TrueMark Amplification Control (Cat. No. A55698) or TrueMark Amplification Control I, low conc. (Cat. No. A55699):
- Added to a single well per target in the real-time PCR plate as a positive control. The recommended concentration for the positive control is 10,000 copies/reaction.
- Monitors real-time PCR reaction setup and reagent integrity.
- Can be ordered separately.
- Includes all the targets in the TrueMark panels.
Negative (extraction) Control:
- It is a negative control on the extraction sample plate that contains water.
- The negative (extraction) control is run on the real-time PCR plate.
- Monitors cross-contamination of reagents during reaction setup.
TaqMan Universal Extraction Control Organism (B. atrophaeus) (Cat. No. A39180):
- Used only for TrueMark Urinary plates.
- Contains three vials of lyophilized Bacillus atrophaeus, for use as a process control for cell lysis and nucleic acid recovery.
- Like other gram-positive bacteria, Bacillus atrophaeus has thick cell walls that can be difficult to lyse. This characteristic makes Bacillus atrophaeus an ideal control to monitor the efficiency of cell lysis and subsequent nucleic acid recovery.
- Is supplied lyophilized with a quantity of 1 x 10E9 copies/vial and is reconstituted in 200 µL of PBS (1X), pH 7.4 to a final concentration of 5 x 10E6 copies/µL.
- The following B. atrophaeus dilution and spike-in process instructions are recommended:
Vortex for 30 seconds, until the pellet is resuspended. Make the suitable number of aliquots and stored at -20 degrees C for up to 4 months. Perform further dilution to obtain the working concentration of 5 x 10E4 copies/µL. 10 µL of the control is added to urine samples in each extraction well at the start of extraction.
It takes approximately 3 hours for running the assay starting from sample preparation to receiving the Ct value.
We recommend running the plate immediately. If the plate is not ready to run, store it at 2-8 degrees C for up to 2 hours.