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Thermo Scientific™ Triton™ X-100 Surfact-Amps™ Detergent Solution

Catalog No. PI85112
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250 mL
6 x 10 mL
1 L
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PI85112 250 mL
PI85111 50 mL
PI28314 6 x 10 mL
PI28313 1 L
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Catalog No. PI85112 Supplier Thermo Scientific™ Supplier No. 85112
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Description

Thermo Scientific Triton X-100 Surfact-Amps Detergent Solution is a highly-purified Triton™ X-100 detergent stabilized as a 10% solution (w/v) in variable sizes packaged under nitrogen in 10 mL glass ampules or three sizes of non-leaching HDPE bottles, ensuring their stability and eliminating the accumulation of peroxides and degradation products.

Features of Triton X-100 Surfact-Amps Detergent Solution:

  • Triton X-100—a nonionic detergent for use in various protein methods
  • Accurate—precise 10% detergent solution in ultrapure water
  • Easy-to-use—solution is simple to dispense and dilute for use
  • Exceptionally pure—less than 1.0μeq/mL peroxides and carbonyls
  • Stable—packaged under inert nitrogen gas in glass ampules or HDPE bottles

Specifications for Triton X-100 Surfact-Amps Detergent Solution:

  • Visual: Clear to slightly hazy, colorless solution, free of particulate matter.
  • Concentration: 10.0±1.0%
  • Oxidants: ≤1.0μeq/mL
  • Carbonyls: ≤1.0μeq/mL
  • Suspended Solids: Residue present must not exceed Residue Reference.

Properties of Triton X-100:

  • Molecular Weight: 647 g
  • Detergent Class: Nonionic polyoxyethylene surfactant
  • Aggregation Number: 140
  • Micelle Molecular Weight: 90,000 g
  • Critical Micelle Concentration (CMC): 0.24 mM (0.0155% w/v)
  • Cloud Point: 64°C
  • Dialyzable: No

Triton X-100 is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins.

Related Products

  • Surfact-Amps™ Detergent Sampler (Cat. No. 28340)

Specifications

Content And Storage Store between 20°C and 25°C
Form Liquid
Product Line Surfact-Amps, Triton
Reagent Type Detergent Solution
Quantity 250 mL

Frequently Asked Questions (FAQs)

I need to do immunolabeling of a thick cryosection, around 60 µm thick. What should I do to ensure good labeling with my antibody?

One thing you should do is to permeabilize well, by including 0.1% to 0.2% Triton X-100 in all washes and incubations. Another recommendation is to extend the labeling times to overnight at 4oC for both primary antibody and secondary antibody. Finally, label the section free-floating, rather than adhered to a slide, to gain penetration for the antibody from both sides.
What are the storage conditions for Triton X-100 Surfact-Amps Detergent Solution (Cat. No. 85111)?

After opening, we recommend storing the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. Frequent opening should be avoided because Triton X-100 is susceptible to autoxidation upon exposure to air.
Can you provide the shelf life for Triton X-100 Surfact-Amps Detergent Solution (Cat. No. 85111)?

Triton X-100 Surfact-Amps Detergent Solution is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (20-25 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

For Research Use Only. Not for use in diagnostic procedures.