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  5. Thermo Scientific™ MagJET Plasmid DNA Kit

Thermo Scientific™ MagJET Plasmid DNA Kit

Catalog No. FERK2791
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4 x 96 Preps
96 Preps
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FERK2791 96 Preps
FERK2792 4 x 96 Preps
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Catalog No. FERK2791 Supplier Thermo Scientific™ Supplier No. K2791
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Description

Thermo Scientific MagJET Plasmid DNA Kit, sufficient for 96 preps, is designed for fast and efficient purification of plasmid DNA (up to 4–5 μg/mL) or high copy plasmid DNA from overnight E. coli culture.

Thermo Scientific MagJET Plasmid DNA Kit, sufficient for 96 preps, is designed for fast and efficient purification of plasmid DNA (up to 4–5 μg/mL) or high copy plasmid DNA from overnight E. coli culture.

The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET Magnetic Beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual purification for low sample throughput users.

The resulting high quality DNA is free of proteins, nucleases and other contaminants or inhibitors. Purified plasmid DNA can be used in a wide range of downstream applications such as PCR, qPCR and other enzymatic reactions.

Highlights
• High yields—up to 5 μg of plasmid DNA from 1 mL of bacterial culture
• Pure—isolated DNA is immediately ready-to-use in downstream application such as sequencing, transcription, digestion with restriction enzymes or transfection of robust cell lines
• Flexible—automation and manual processing protocols available
• Repeatable results and robust performance

Applications
Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
• In vitro transcription
• Transformation
• Transfection of robust cell lines

Specifications

Format Kit
Isolation Technology Magnetic Bead
Sample Type Bacteria
For Use With (Application) Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
High-throughput Compatibility High-throughput Compatible, Not High-throughput Compatible (Manual)
Prep Scale < 100 μg (Small-Scale) Plasmid DNA
Product Line MagJET
Product Type Plasmid DNA Kit
Quantity 96 Preps
Target Plasmid DNA

Frequently Asked Questions (FAQs)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.
Are MagJET and GeneJET plasmid purification kits compatible with EndA+ bacterial strains?

Yes, MagJET and GeneJET Plasmid DNA Purification Kits are suitable for plasmid DNA purification from EndA+ strains. Please refer to the specific kit protocol for details. GeneJET Plasmid Miniprep Kit requires Wash Solution I (Cat. No. R1611, not included in the kit) for EndA+ strains.
Which magnetic rack should I use to follow the manual protocol of MagJET kits?

Thermo Scientific offers MagJET Separation Rack of different formats for 1.5 mL tubes (Cat. Nos. MR01, MR02), 50 mL tubes (Cat. No. MR04), and 96-well plates (Cat. No. MR03). You may use other compatible magnetic racks available in the market as well.
Is a sample size available for MagJET kits?

No, we do not offer a sample size for MagJET kits.
Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.
Can I use the KingFisher instruments for plasmid purification?

We recommend using the MagJet Plasmid DNA kit (Cat. No. K2791) for plasmid purification. Scripts for using the MagJet Plasmid DNA kit with the KingFisher Flex and KingFisher Duo Prime Purification Systems are available for download on the kit product page (https://www.thermofisher.com/order/catalog/product/K2791). The ChargeSwitch Plasmid ER Mini Kit (Cat. No. CS10100) is also an option for plasmid purification. There is no script currently available for using ChargeSwitch Plasmid ER Mini Kit, but you can create a custom protocol for use with the KingFisher Flex and KingFisher Duo Prime Purification Systems.
Can I edit the manufactured KingFisher Flex protocols?

No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.
Can I use magnetic beads from other vendors with the KingFisher Flex instrument?

For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.

I am working with liquid samples with high volume and want to extract nucleic acid from them using the KingFisher Flex instrument. Can I use more volume than the protocol advises?

Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.

Can I scale up my reaction volume with the KingFisher Flex instrument?

Yes, reaction volumes can be scaled up, and all other volumes (i.e. wash volumes) will have to be increased by the same fold difference. It is generally not recommended to scale up reaction volumes more than 2-fold unless the plate's volume capacity will allow it.

For Research Use Only. Not for use in diagnostic procedures.