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Thermo Scientific™ Luminaris HiGreen qPCR Master Mix
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.
Supplier: Thermo Scientific™ K0992
Includes: Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; Nuclease-Free Water
Description
Ready-to-use qPCR master mixes with premixed UDG optimized for SYBR Green chemistry using standard cycling protocol. Master mixes available with or without blue color.
- UDG in the master mix to prevent carry-over contamination
- Equal performance of blue and colorless master mixes
- Versions with premixed ROX or fluorescein for different real-time platforms
- Standard cycling protocols
- Excellent qPCR performance
- Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity: single copy detection
- Wide linear range: accurate quantification across 9 orders of magnitude
- Reproducibility and convenience: ready-to-use 2X master mix
Chemical Identifiers
| 2X | |
| High | |
| Taq DNA Polymerase | |
| BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene | |
| qPCR Master Mix | |
| 2 X |
| SYBR | |
| qPCR | |
| Standard | |
| Luminaris | |
| Gene Expression | |
| DNA, dsDNA |
Specifications
| 2X | |
| SYBR | |
| qPCR | |
| Standard | |
| Luminaris | |
| 500 reactions | |
| Gene Expression | |
| 500 Reactions |
| Contains: • 4 x 1.25 mL Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 500 x 20 μL reactions • 4 x 1.25 mL Nuclease-Free Water Store at -20°C. |
|
| High | |
| Taq DNA Polymerase | |
| BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene | |
| qPCR Master Mix | |
| 500 Reactions | |
| 2 X | |
| DNA, dsDNA |
For Research Use Only. Not for use in diagnostic procedures.