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  7. Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, low ROX

Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, low ROX

Catalog No. FERK0974
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No. of Reactions:
1250 Reactions
250 Reactions
500 Reactions
5000 Reactions
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Catalog No. No. of Reactions
FERK0974 5000 Reactions
FERK0972 500 Reactions
FERK0971 250 Reactions
FERK0973 1250 Reactions
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Catalog No. FERK0974 Supplier Thermo Scientific™ Supplier No. K0974
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Description

Includes

Luminaris HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer. The master mix contains low concentration of ROX passive reference dye for specific real-ti

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: single copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix

Specifications

Concentration 2X
Content And Storage Contains:
• 4 x 12.5 mL Luminaris HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 5000 x 20 μL reactions. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms.
• 2 x 30 mL Nuclease-Free Water

Store at -20°C.
Detection Method SYBR
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Product Line Luminaris
Product Type qPCR Master Mix
Quantity 5,000 reactions
Sufficient For 5000 Reactions
For Use With (Application) Gene Expression
Fidelity (vs. Taq) 2 X
No. of Reactions 5000 Reactions
Sample Type DNA, dsDNA
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For Research Use Only. Not for use in diagnostic procedures.