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  7. Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, fluorescein

Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, fluorescein

Catalog No. p-4861497
Catalog No. FERK0984 Supplier Thermo Scientific™ Supplier No. K0984

Description

Includes

Luminaris HiGreen Fluorescein qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer. The master mix contains fluorescein passive reference dye for specific real-time platfo

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: single copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix

Specifications

Concentration 2X
Content And Storage Contains:
• 4 x 12.5 mL Luminaris HiGreen Fluorescein qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 5000 x 20 μL reactions. The master mix contains fluorescein passive reference dye for specific real-time platforms.
• 2 x 30 mL Nuclease-Free Water

Store at -20°C.
Detection Method SYBR
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
Product Line Luminaris
Product Type qPCR Master Mix
Sufficient For 5000 Reactions
For Use With (Application) Gene Expression
Fidelity (vs. Taq) 2 X
No. of Reactions 5000 Reactions
Sample Type DNA, dsDNA
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