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Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix DFS Item

Single-tube, 4X RT-qPCR master mix that provides for sensitive, reproducible detection of up to four different RNA (or DNA) targets in a single multiplex reaction.

Manufacturer:  Applied Biosystems™ LSA28525

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Catalog No. A28525



High sensitivity:

  • To enable reproducible detection of low-titer pathogen and transcripts, TaqPath One-Step Multiplex Master Mix has been optimized as a higher-concentration 4X master mix that allows you to input more sample into each reaction, increasing sensitivity even in low-volume reactions

Wide dynamic range compatible with RNA and DNA:

  • TaqPath One-Step Multiplex Master Mix has been optimized to provide high specificity and dynamic range for use with both RNA and DNA targets
  • Since virology labs often test for both RNA and DNA viruses, TaqPath One-Step Multiplex Master Mix is designed to use a single protocol to assay both types of nucleic acid
  • This input flexibility can help streamline the number of different workflows in your lab to improve efficiency

Optimized for multiplexing:

  • TaqPath One-Step Multiplex Master Mix has been validated for multiplexing up to 4 targets simultaneously, allowing for additional targets and/or controls to be run simultaneously for efficiency or quality control purposes
  • Rather than including ROX dye as a passive reference, the TaqPath 1-step Multiplex Master Mix includes the longer emission wavelength MUSTANG PURPLE dye as a passive reference
  • This longer wavelength passive reference dye enables measurement of the JUN dye, or a similar emission wavelength dye, in the channel previously used to measure ROX dye

Inhibitor tolerant:

  • Unlike other master mixes on the market, TaqPath One-Step RT-qPCR Master Mix's unique proprietary formulation allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples
  • TaqPath master mixes demonstrate increased tolerance of inhibitors compared to competitor mixes

Manufacturing production and process controls:

  • TaqPath One-Step Multiplex Master Mix is manufactured under a strong ISO13485 quality management system that uses traceable quality raw materials and validated operating procedures
  • With established controls from purchasing through QC release, TaqPath product manufacturing is designed to produce consistent product performance lot after lot

Broad instrument compatibility:

  • The TaqPath One-Step Multiplex Master Mix can be run in either fast or standard cycling conditions with equivalent performance across a wide variety of Applied Biosystems™ qPCR platforms, including the 7500, 7500 Fast, 7500 Fast Dx, QuantStudio™ 5, 6, 7, 12k, Dx, and ViiA™ 7 real-time PCR systems

General purpose reagent:

  • TaqPath One-Step RT-qPCR Master Mix is a General Purpose Reagent (GPR), labeled for laboratory use


Real Time PCR (qPCR)
0.5 mL
100 Reactions of 20 μL
Primer-Probe Detection
Fast DNA Polymerase
7500 Fast System, 7500 System, QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, ViiA™ 7 System, QuantStudio™ Dx, 7500 Fast Dx System
qPCR Master Mix
Dry Ice
1-Step qRT-PCR
Fast or Standard
0.5 mL
Product Certifications