Applied Biosystems™ TaqMan™ RNA-to-C_T™ 1-Step Kit 

Description

The TaqMan™ RNA-to-CT™ One-Step Kit is recommended for a variety of applications including general gene expression studies, biomarker discovery, and microarray validation.

• Accurate across a wide dynamic range for dependable performance
• High sensitivity, enabling low copy number detection and precise quantification
• Validated with TaqMan™ Gene Expression Assays for easy experimental setup
• Consistent performance with a wide variety of targets, including AT-rich, GC-rich and long sequences
• Fewer pipetting steps means less hands-on-time and less chance for errors

Efficiency across a Broad Range of Input Concentrations

• Maximum flexibility in RNA template input quantity requires optimal real-time PCR efficiency across a broad range of template quantities
• The result is comparable to that of the TaqMan™ RNA-to-CT™ 2-Step Kit, and is a significant improvement in dynamic range and sensitivity over the TaqMan™ One-Step RT-PCR Master Mix Reagents.

High Sensitivity at Low Target Concentration

• The sensitivity of TaqMan™ RNA-to-CT™ One-Step Kit was validated using a synthetic RNA template for which copy number is precisely known
• Significant sampling error occurs when measuring low quantities of target, so statistical analysis is required for proper evaluation using multiple replicates
• Statistical analysis indicates high confidence of sample quantification based on a T-test, consistent with as few as 10 copies of target.

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