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Applied Biosystems™ TaqMan™ Pri-miRNA Assay
Description
TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold-standard assay performance and data quality for pri-miRNA studies.
• Highly specific—quantitiate miRNA transcription from a single genomic locus
• Fast, simple, and scalable—two-step RT-qPCR provides high-quality results
• A new perspective—measure miRNA expression at the gene level
TaqMan Pri-miRNA Assays apply the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan genomic assays are renowned to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.
Design and selection
Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan Pri-miRNA Assay and TaqMan MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.
Pre-formulated assay (20X; 60X mix)
• 2 unlabeled PCR primers (900 nM each final 1X reaction concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X reaction concentration)
Specifications
Specifications
| Concentration | 20X |
| Content And Storage | 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers). Store at -15 to -25°C. |
| Format | Tube |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Reaction Speed | Fast |
| Product Line | TaqMan |
| Product Type | Pri-miRNA Assay |
| Quantity | M (750 reactions), made to order |
| Shipping Condition | Room Temperature |
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Frequently Asked Questions (FAQs)
TaqMan Pri-miRNA Assays are designed to detect the transcript that contains the targeted stem loop. In some instances, e.g. when stem-loop sequences are clustered together and are transcribed on the same transcript, a TaqMan Pri-miRNA Assay targeting one of the stem-loops will also detect the other stem loops in the cluster.
Yes. The specific stem-loop chemistry of the TaqMan MicroRNA Assay and its design prevent amplification of the pri-form. Vice versa, the chemistry of the TaqMan Pri-miRNA Assays is not amenable to amplify the mature form. In particular cases, assays designed to detect mature miRNAs derived from the 3' arm of the stem loop may also detect pre-miRNA.
TaqMan endogenous control assays are compatible for pri-miRNA (visit the TaqMan Endogenous Control Assays web portal for more information).
We recommend the following kits:
- High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4374966).
- High-Capacity RNA-to-cDNA Kit (Cat. No. 4387406).
For Research Use Only. Not for use in diagnostic procedures.