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  7. Applied Biosystems™ TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)

Applied Biosystems™ TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)

Catalog No. 4488593
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200 Reactions
40 Reactions
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Catalog No. No. of Reactions
4488593 200 Reactions
4391128 40 Reactions
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Catalog No. 4488593 Supplier Applied Biosystems™ Supplier No. 4488593
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Description

TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample.

TaqMan™ PreAmp Master Mix lets you: • Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE) • Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan™ Gene Expression assays • Analyze up to 100 gene expression targets with minimal hands-on time Preserves Equilibrium of Targets Without Preamplification Bias: In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan™ PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA. Easy Workflow: With TaqMan™ PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan™ assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan™ PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp™ 9700 thermal cycler. For Research Use Only. Not for use in diagnostic procedures.

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

Content And Storage Contains one 5-mL bottle of 2X master mix.

Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method Primer-probe
Format Bottle
GC-Rich PCR Performance High
PCR Method qPCR
Reaction Speed Standard
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Product Line TaqMan
Product Type PreAmp Master Mix
Quantity 200 reactions
Sufficient For 200 Reactions
For Use With (Application) Pre-amplification
No. of Reactions 200 Reactions
Sample Type cDNA
Volume 5 mL
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Frequently Asked Questions (FAQs)

Can I use preamplification with the TaqMan SNP Genotyping Assays?

There is a preamplification protocol included with our TaqMan Sample-to-SNP Kit. This is designed to work with a lysate sample.
How does the SuperScript IV Single Cell/Low Input cDNA PreAmp Kit differ from TaqMan PreAmp Master Mix?

TaqMan PreAmp Master Mix is designed for targeted preamplification of up to 100 targets from small amounts of cDNA, while SuperScript IV Single Cell/Low Input cDNA PreAmp Kit allows global preamplification of full-length cDNA from single cells or low input RNA. It provides reagents for cell lysis, reverse transcription and preamplification steps.
How do I reorder the TaqMan PreAmp Master Mix, Cat No. 4384266? It does not match the Cat. No. when I search online.

This is an internal part number for the TaqMan PreAmp Master Mix. To order, please use Cat No. 4391128 for the 40 reaction size or Cat. No. 4488593 for the 200 reaction size. This is the same product.

For Research Use Only. Not for use in diagnostic procedures.