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Applied Biosystems™ TaqMan™ Array Human Protein Kinase Pathways

Catalog No. 4414076
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Description

TaqMan™ Array Human Protein Kinase Pathways 96-well Plate contains 92 assays to protein kinases and associated genes and 4 assays to candidate endogenous control genes.

The panel of assays in this plate targets kinases from the receptor protein-tyrosine kinase (RPTK) families and from the Serine/Threonine kinase families. Also included, are non-kinase genes involved in signal transduction and mediate protein-protein interaction, transcriptional regulation, neural development and cell adhesion. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment.

TaqMan Array Plates contain the same TaqMan Gene Expression Assays that have been available for years in a convenient, dried, 96-well format. Extensive tests have found no difference in performance between TaqMan Assays supplied in the liquid format and assays dried into a TaqMan Array Plate.

Ideal for projects such as validation of microarray and RNAi data, and pathway studies, the plates include enough TaqMan Assay in each well for one 20μL reaction.

Each pack of TaqMan Array Plates is shipped with a CD containing the plate map along with the details of each TaqMan Assay. This information is imported into the real-time PCR instrument to enable proper labeling of experimental data and easy access to Assay ID numbers for simple reordering.

  • Affordable; minimum order of three plates; ideal for small, medium or large-sized projects
  • Fast delivery; on your bench in days
  • Convenient; dried PCR primer and TaqMan probe sets supplied in a 96- well plate; just add master mix and sample
  • TaqMan Array Plates have been optimized for use with TaqMan Gene Expression Master Mix, but are also compatible with TaqMan Universal PCR Master Mix
  • For studies using cultured cells, TaqMan Array Plates are also compatible (and recommended) with the TaqMan Gene Expression Cells-to-Ct™ Kit
  • TaqMan Array Plates must be run on a real-time PCR instrument with 96-well plate capability
  • The plates have been validated for use on Applied Biosystems™ 7000, 7300, 7500 and 7900HT Fast Real-Time PCR Systems using standard 96-well blocks, standard PCR cycling conditions, and 20μL reaction

Specifications

Content And Storage Each TaqMan™ Array Plate well contains a single TaqMan™ Assay for one 20 μL reaction. The plates can be stored at room temperature or at 4°C.
Form Dried Down
Format 96-well plate
Reaction Speed Standard
Internal Probe Modification MGB (Minor Groove Binder) (3'), Non-Fluorescent Quencher (3'), FAM (5')
Label or Dye FAM
Product Line TaqMan
Purification Method Standard
Quantity 1 standard plate
Research Category Cell Cycle & Proliferation, Apoptosis, Neurobiology, Signal Transduction, Biomarker-Related Pathway(s), Development, Reproduction, Immunology, Oncology
Species Human
For Use With (Application) Gene Expression
No. of Reactions 1 x 20 μL Reactions
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Frequently Asked Questions (FAQs)

What is the minimum amount of TaqMan array plates I need to purchase to submit an order?

The minimum amount of TaqMan Array plates needed to submit an order is based on the plate type:
• Inventoried TaqMan array plates: 1 plate
• TaqMan Flexible Content panel array plates: 6 plates
• Custom TaqMan array plates: 6 plates
• Specialty TaqMan array plates: 10-20 plates (depends on assay format and configuration). Please contact our Specialty Plates team at Specialty_Plates@thermofisher.com for details.
Can our TaqMan Array Plates be run on non-Applied Biosystem real-time PCR instruments?

We recommend using our Plastics Selection Tool to verify which plates are compatible with your instrument model, https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/plastics-selection-guide.html.

Based on our testing, the 96-well TaqMan Array Plates can be used on the following non-Applied Biosystem real-time PCR instruments:
• Standard (0.2mL) 96-well Plates: Bio-Rad IQ5
• Fast (0.1mL) Plates: Bio-Rad CFX Connect and Bio-Rad CFX96 Touch
How do I obtain TaqMan documentation files for my TaqMan array plates?

Beginning in April 2016, TaqMan documentation files for TaqMan Array Plates (assay information files (AIF), experimental setup files, and plate layout files) can be downloaded from the Thermo Fisher Scientific website at https://www.thermofisher.com/taqmanfiles. To obtain your TaqMan documentation files:
• Select the appropriate product type.
• Enter the information being requested for the product type.
• Fixed TaqMan array plates: Enter the Part number and Batch number
• TaqMan Flexible Content panel array plates/Custom TaqMan array plates: Enter the Sales Order number and Batch number
• Click Submit.

For Research Use Only. Not for use in diagnostic procedures.