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Applied Biosystems™ TaqMan™ Array Human Endogenous Controls Plate, Fast 96-well

Catalog No. 4426700
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Description

TaqMan™ Array Human Endogenous Controls Plate, Fast 96-well

These 96-well plates are pre-configured with the most appropriate TaqMan™ Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan™ Array Human Endogenous Control Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and⁄or whole genome microarray tests carried out on numerous human tissues. They have been shown to be expressed constitutively and at moderate abundance across most test samples.

Specifications

Content And Storage Control Primer-Probe Set(s)
Form Dried Down
Format 96-well plate
Reaction Speed Fast
Internal Probe Modification MGB (Minor Groove Binder) (3'), Non-Fluorescent Quencher (3'), FAM (5')
Label or Dye FAM
Product Line TaqMan
Quantity 1 x 96 well plate
Species Human
For Use With (Application) Gene Expression
No. of Reactions 1 x 10 μL Reactions
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Frequently Asked Questions (FAQs)

What mastermix can I use for running samples on TaqMan Gene Signature Array Plates?

We recommend that for standard (20 µL) TaqMan Array Plates, you use either the Universal Master Mix (I or II) or the Gene Expression MasterMix. For fast (10 µL) TaqMan Array Plates, use the Fast Universal Master Mix. The Fast Advanced Master Mix can also be used for either plate type, but we recommend following the protocol in this manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025706_TaqManFastAdvMM_UG.pdf).
What is the minimum amount of TaqMan array plates I need to purchase to submit an order?

The minimum amount of TaqMan Array plates needed to submit an order is based on the plate type:
• Inventoried TaqMan array plates: 1 plate
• TaqMan Flexible Content panel array plates: 6 plates
• Custom TaqMan array plates: 6 plates
• Specialty TaqMan array plates: 10-20 plates (depends on assay format and configuration). Please contact our Specialty Plates team at Specialty_Plates@thermofisher.com for details.
Can our TaqMan Array Plates be run on non-Applied Biosystem real-time PCR instruments?

We recommend using our Plastics Selection Tool to verify which plates are compatible with your instrument model, https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/plastics-selection-guide.html.

Based on our testing, the 96-well TaqMan Array Plates can be used on the following non-Applied Biosystem real-time PCR instruments:
• Standard (0.2mL) 96-well Plates: Bio-Rad IQ5
• Fast (0.1mL) Plates: Bio-Rad CFX Connect and Bio-Rad CFX96 Touch
How do I obtain TaqMan documentation files for my TaqMan array plates?

Beginning in April 2016, TaqMan documentation files for TaqMan Array Plates (assay information files (AIF), experimental setup files, and plate layout files) can be downloaded from the Thermo Fisher Scientific website at https://www.thermofisher.com/taqmanfiles. To obtain your TaqMan documentation files:
• Select the appropriate product type.
• Enter the information being requested for the product type.
• Fixed TaqMan array plates: Enter the Part number and Batch number
• TaqMan Flexible Content panel array plates/Custom TaqMan array plates: Enter the Sales Order number and Batch number
• Click Submit.
Can poly A+ purified RNA samples be used with the TaqMan Human Endogenous Control Plate (Cta. No. 4309199)?

No. The plate has been designed to evaluate only total RNA. Ribosomal RNA will be removed from poly A+ purified RNA samples, and is required for accurate detection of the 18s rRNA endogenous control assay used on the plate.

For Research Use Only. Not for use in diagnostic procedures.