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  5. Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

Catalog No. PI37074
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100 mL Kit
250 mL Kit
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PI37074 250 mL Kit
PI37075 100 mL Kit
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Catalog No. PI37074 Supplier Thermo Scientific™ Supplier No. 37074
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Description

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

Thermo Scientific™ SuperSignal ELISA Femto Maximum Sensitivity Substrate is formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

SuperSignal ELISA Femto Substrate generates detectable chemiluminescence (light) within one minute of addition to trace amounts of soluble HRP, saving up to 30 minutes per assay compared to colorimetric ELISA substrates. This feature is ideal for high-throughput screening (HTS) applications in which as many as 100,000 assays can be performed daily on robotic equipment. Because incubation periods are often a limiting factor in the development of rapid automated assays, SuperSignal ELISA Femto Substrate is the logical choice for automated HTS applications.

Highlights:

  • Immediate light generation – intense signal generated immediately at both room temperature and 37°C

  • Improved low-end linearity – easy detection of low quantities of proteins with high signal-to-noise ratios and low-end linearity of dose response curves

  • High sensitivity – femtogram-level detection of target proteins

  • Reduction in assay time – high sensitivity allows for reduction in incubation steps

  • Stability – storage for six months at room temperature or a minimum of 12 months at 4°C with a six-hour working solution stability

  • Emits light at 425nm – measurable by luminometers

Includes:

Luminol/Enhancer Solution; Peroxide Buffer

Specifications

Product Type ELISA Substrate
Quantity 250 mL Kit
Detection Method Chemiluminescence
Product Line SuperSignal
Substrate Properties Chemical Substrate
Substrate Type HRP (Horseradish Peroxidase)
Content And Storage Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature.

Frequently Asked Questions (FAQs)

What type of instrument do I use to read the plates detected with the SuperSignal ELISA Femto Maximum Sensitivity Substrate?

A luminometer is used to measure relative light units.
What microplates work best with the SuperSignal ELISA Femto Maximum Sensitivity Substrate?

White plates provide the best signal-to-noise ratio. Black plates provide lower background but less detectable signal. Here are two suggestions we offer for white plates:

-Pierce 96-Well Polystyrene Plates, White Opaque (Cat. No. 15042)
-Nunc FluoroNunc/LumiNunc 96-Well Plates (Cat. No. 437796)

What signal duration occurs with SuperSignal ELISA Femto Maximum Sensitivity Substrate?

Maximum signal is reached within two minutes and then declines steadily until it is neglible after twenty minutes.
How sensitive is SuperSignal ELISA Femto Maximum Sensitivity Substrate?

Our researchers have detected down to 168 fg of interleukin-2 in sandwich ELISA.
Can I dilute the SuperSignal ELISA Femto Maximum Sensitivity Substrate working reagent if my signal is too intense?

We do not recommend diluting the working reagent. It is better to dilute or otherwise optimize primary and or secondary antibody concentrations or use more washes between incubation steps.
At what wavelength is SuperSignal ELISA Femto Maximum Sensitivity Substrate measured?

The light emission wavelength is measured at 425 nm.
Have you tested the SuperSignal ELISA Femto Substrate with magnetic beads?

We have not tested the SuperSignal ELISA Femto Substrate with magnetic beads. However, we know that some metals can interfere with the substrate. Therefore, depending on the chemistry of the beads, they may or may not interfere with the substrate.
Is the time taken to add your SuperSignal ELISA Femto Substrate to the wells of a microplate important?

Yes. The signal from the SuperSignal ELISA Femto Substrate ramps up very quickly and optimal signal production lasts only for 5-10 min. Therefore, we recommend using a multichannel pipette to add the substrate to the wells, mix for 1 min, and then read the signal produced. Typically, reading each well for 0.1-1 sec will provide good signal with this substrate.
Note: With the SuperSignal ELISA Pico Chemiluminescent Substrate (Cat. No. 37070, 37069), the signal does not decay as quickly and researchers typically have up to 30 min to read the plate.

For Research Use Only. Not for use in diagnostic procedures.