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Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit DFS Item

Optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in cloning, PCR, and qPCR.

$1,028.00 - $8,130.00

Specifications

Detection Method Fluorescence
Final Product Type First-Strand cDNA
Format cDNA Synthesis Kit
GC-Rich PCR Performance High
PCR Method RT-PCR, RT-qPCR
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Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity  
Catalog Number Mfr. No. No. of Reactions Price Quantity  
11750150 DFS Item
View Documents Promotion Details
Invitrogen™
11750150
50 Reactions
Each for $1,028.00
 
11750350 DFS Item
View Documents Promotion Details
Invitrogen™
11750350
500 Reactions
Each for $8,130.00
 
Description

Description

Features of the SuperScript™ IV kit include:

  • Compatible with a wide range of mammalian cell types grown under different treatment conditions
  • Single-tube format minimizes reagent loss, sample loss, and handling time
  • Total lysate volume can be used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts
  • High-quality cDNA generated for use in a variety of applications, including cloning, PCR, and qPCR
  • Simple protocol takes around 37 minutes
  • Compatible with TaqMan™ and SYBR Green qPCR master mixes from various manufacturers
  • Optimized performance for 1–10,000 cells per sample; results equivalent to those from purified RNA

The SuperScript™ IV CellsDirect cDNA Synthesis Kit includes SuperScript™ IV Reverse Transcriptase. Features of this enzyme include:

  • Reduced RNase H activity
  • Higher thermal stability
  • High yields of cDNA in the first-strand synthesis reaction, for greater sensitivity and enhanced detection of rare transcripts
  • Superior tolerance to various RT inhibitors

SuperScript™ IV reverse transcriptase is a top choice for all RT-PCR and qRT-PCR applications. It is a proprietary MMLV mutant with superior robustness and reliability in RT reactions. It is significantly improved over SuperScript™ III reverse transcriptase in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of the previous enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity.

How it works
In traditional RT-PCR, RNA is first isolated from cells in a time-consuming procedure that can lead to a loss of material. Using the SuperScript™ IV CellsDirect cDNA Synthesis Kit, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss. DNase I treatment is an optional step and can be used to eliminate genomic DNA prior to first-strand synthesis. This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell.

Specifications

Specifications

Fluorescence
cDNA Synthesis Kit
RT-PCR, RT-qPCR
10 min.
Cells
Less Hazardous, Less Waste
SuperScript IV
Dry Ice
First-Strand cDNA
High
Premixed Components
Reverse Transcription
Reverse Transcription
50°C
Reduced
RT-PCR (Endpoint), Real-Time PCR
SDS
Product Certifications