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Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit 
Optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in cloning, PCR, and qPCR.
$1,028.00 - $8,130.00
Specifications
| Detection Method | Fluorescence |
|---|---|
| Final Product Type | First-Strand cDNA |
| Format | cDNA Synthesis Kit |
| GC-Rich PCR Performance | High |
| PCR Method | RT-PCR, RT-qPCR |
Description
Features of the SuperScript™ IV kit include:
- Compatible with a wide range of mammalian cell types grown under different treatment conditions
- Single-tube format minimizes reagent loss, sample loss, and handling time
- Total lysate volume can be used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts
- High-quality cDNA generated for use in a variety of applications, including cloning, PCR, and qPCR
- Simple protocol takes around 37 minutes
- Compatible with TaqMan™ and SYBR Green qPCR master mixes from various manufacturers
- Optimized performance for 1–10,000 cells per sample; results equivalent to those from purified RNA
The SuperScript™ IV CellsDirect cDNA Synthesis Kit includes SuperScript™ IV Reverse Transcriptase. Features of this enzyme include:
- Reduced RNase H activity
- Higher thermal stability
- High yields of cDNA in the first-strand synthesis reaction, for greater sensitivity and enhanced detection of rare transcripts
- Superior tolerance to various RT inhibitors
SuperScript™ IV reverse transcriptase is a top choice for all RT-PCR and qRT-PCR applications. It is a proprietary MMLV mutant with superior robustness and reliability in RT reactions. It is significantly improved over SuperScript™ III reverse transcriptase in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of the previous enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity.
How it works
In traditional RT-PCR, RNA is first isolated from cells in a time-consuming procedure that can lead to a loss of material. Using the SuperScript™ IV CellsDirect cDNA Synthesis Kit, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss. DNase I treatment is an optional step and can be used to eliminate genomic DNA prior to first-strand synthesis. This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell.
Specifications
| Fluorescence | |
| cDNA Synthesis Kit | |
| RT-PCR, RT-qPCR | |
| 10 min. | |
| Cells | |
| Less Hazardous, Less Waste | |
| SuperScript IV | |
| Dry Ice |
| First-Strand cDNA | |
| High | |
| Premixed Components | |
| Reverse Transcription | |
| Reverse Transcription | |
| 50°C | |
| Reduced | |
| RT-PCR (Endpoint), Real-Time PCR |