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Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissues

Catalog No. pi87790
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Catalog No. Quantity
PI87790 1 Kit
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Catalog No. PI87790

Supplier: Thermo Scientific™ LSG87790

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Description

Fractionate tissue sample-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins within 2 hours.

The Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissue includes all reagents and tissue strainers required to perform cell fractionation and enrichment of proteins from five different cellular compartments within 2 hours.

The complete Subcellular Protein Fractionation Kit for Tissue includes a unique 250μm tissue strainer to facilitate rapid separation of cell suspension from tissue debris after homogenization. The optimized reagents enable subsequent stepwise extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from the recovered cells with less than 15% cross-contamination. Tissue extracts obtained with this subcellular fractionation kit are compatible with a variety of downstream applications, including Western blotting, protein quantitation, electrophoretic mobility shift, reporter-gene and enzyme-activity assays.

Highlights:

  • Optimized formulations and protocols specific for fractionation of tissue

  • Complete obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit

  • Time efficient extract functional protein fractions without ultracentrifugation in approximately 2 hours

  • Convenient unique tissue strainer allows rapid removal of tissue debris

  • Compatible use extracts for downstream applications such as protein assays, Western blotting, gel-shift assays and enzyme-activity assays

  • Versatile extract proteins from a variety of tissue types, including liver, heart, brain, kidney, lung and spleen

Recommended for:

Obtain cytoplasmic, soluble nuclear, chromatin-bound, membrane and cytoskeletal proteins from the same tissue sample; Profile disease-state versus normal tissues for proteins from the cytoplasm, nucleus, membrane and cytoskeleton; Study protein translocation in tissues

Specifications

Product Type Subcellular Protein Fractionation Kit
Content And Storage Upon receipt store kit at -20°C or store individual components as indicated above. Kit is shipped with dry ice.
Form Liquid
Buffer Tissue Extraction Buffer, Lysis Buffer
Reagent Type Tissue Extraction Buffer, Detergent Solution, Cell Lysis Buffer, Subcellular Fractionation
Sufficient For 25 Samples
Quantity 1 Kit