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Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissues

Catalog No. PI87790
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Catalog No. PI87790 Supplier Thermo Scientific™ Supplier No. LSG87790
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Description

Fractionate tissue sample-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins within 2 hours.

The Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissue includes all reagents and tissue strainers required to perform cell fractionation and enrichment of proteins from five different cellular compartments within 2 hours.

The complete Subcellular Protein Fractionation Kit for Tissue includes a unique 250μm tissue strainer to facilitate rapid separation of cell suspension from tissue debris after homogenization. The optimized reagents enable subsequent stepwise extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from the recovered cells with less than 15% cross-contamination. Tissue extracts obtained with this subcellular fractionation kit are compatible with a variety of downstream applications, including Western blotting, protein quantitation, electrophoretic mobility shift, reporter-gene and enzyme-activity assays.

Highlights:

  • Optimized formulations and protocols specific for fractionation of tissue

  • Complete obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit

  • Time efficient extract functional protein fractions without ultracentrifugation in approximately 2 hours

  • Convenient unique tissue strainer allows rapid removal of tissue debris

  • Compatible use extracts for downstream applications such as protein assays, Western blotting, gel-shift assays and enzyme-activity assays

  • Versatile extract proteins from a variety of tissue types, including liver, heart, brain, kidney, lung and spleen

Recommended for:

Obtain cytoplasmic, soluble nuclear, chromatin-bound, membrane and cytoskeletal proteins from the same tissue sample; Profile disease-state versus normal tissues for proteins from the cytoplasm, nucleus, membrane and cytoskeleton; Study protein translocation in tissues

Specifications

Product Type Subcellular Protein Fractionation Kit
Content And Storage Upon receipt store kit at -20°C or store individual components as indicated above. Kit is shipped with dry ice.
Form Liquid
Buffer Tissue Extraction Buffer, Lysis Buffer
Reagent Type Tissue Extraction Buffer, Detergent Solution, Cell Lysis Buffer, Subcellular Fractionation
Sufficient For 25 Samples
Quantity 1 Kit

Frequently Asked Questions (FAQs)

Can you provide the shelf-life for the Subcellular Protein Fractionation Kit for Tissues?

The Subcellular Protein Fractionation Kit for Tissues is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
What kits do you offer for cell fractionation?

We offer the following kits for cell fractionation:

Mem-PER Plus Membrane Protein Extraction Kit, Cat. No. 89842
NE-PER Nuclear and Cytoplasmic Extraction Reagents, Cat. No. 78835
Subcellular Protein Fractionation Kit for Cultured Cells, Cat. No. 78840
Subcellular Protein Fractionation Kit for Tissues, Cat. No. 87790
Mitochondrial Isolation Kit for Cultured Cells, Cat. No. 89874
Mitochondrial Isolation kit for Tissue, Cat. No. 89801
Lysosome Enrichment Kit for Tissues and Cultured Cells, Cat. No. 89839
Why is cell fractionation important?

Isolation of subcellular fractions and concentration of low-abundantce proteins allows for more efficient identification and study of the proteins of interest.