missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Modifying Enzymes
  5. Thermo Scientific™ RiboLock RNase Inhibitor (40 U/μL)

Thermo Scientific™ RiboLock RNase Inhibitor (40 U/μL)

Catalog No. FEREO0381
Change view
Click to view available options
Quantity:
2,500 units
24 x 2,500 units
4 x 2,500 units
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
FEREO0381 2,500 units
FEREO0382 4 x 2,500 units
FEREO0384 24 x 2,500 units
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Catalog No. FEREO0381 Supplier Thermo Scientific™ Supplier No. EO0381
Only null left

Description

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.

RiboLock RNase Inhibitor inhibits the activity of RNases A , B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.

  • Does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H
  • Performs under a wide range of reaction conditions
  • Protects RNA from degradation at temperatures up to 55°C
Quality Control:
  • The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases and ribonucleases confirmed by appropriate quality tests
  • Functionally tested in RNA and cDNA synthesis
Source:
  • E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor
Molecular Weight:
  • 49.6kDa monomer Definition of Activity Unit:
    • One unit of the RiboLock RNase Inhibitor inhibits the activity of 5ng RNase A by 50%
    • Inhibitor activity is assayed in the following mixture: 100mM Tris-HCl (pH 7.5), 1.2mM EDTA, 0.1mg/mL BSA, 100ng/mL RNase A, 0.1mg/mL [3H]-RNA, 50mg/mL yeast RNA, 8mM DTT
    Storage Buffer:
    • The enzyme is supplied in: 20mM HEPES-NaOH (pH 7.5), 50mM NaCl, 8mM DTT, 0.5mM ELUGENT Detergent and 50% (v/v) glycerol
    Inhibition and Inactivation
    • Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound
    • Inactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min. heating at 70°

    Recommended for:

    • Inhibition of RNA degradation in the following: in vitrotranscription (1), cDNA synthesis (2), in vitrotranslation (3), isolation of mammalian cell fractions that contain mRNA-protein complex
    • RNA purification and storage
    • Separation and identification of specific ribonuclease activities
    • Studies of tumor suppression

    Note:

    • DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity
    • Recommended concentration 1u/μl of a reaction mixture

Specifications

Concentration 40 U/μL
Compatible Buffer Storage Buffer
Quantity 2,500 units
Product Type RNase Inhibitor
Product Line RiboLock

Frequently Asked Questions (FAQs)

What are the recommended reaction time and temperature for inactivating RNAse A with Thermo Scientific RiboLock RNase Inhibitor?

The “activity” of RiboLock RNAse Inhibitor in principle is temperature-independent because there are no chemical reactions involved. At both 25 degrees C and 37 degrees C, the same quantity of RiboLock RNAse Inhibitor is needed to quench a given amount of RNase A.
What is the molecular weight of Thermo Scientific RiboLock RNase Inhibitor?

The molecular weight of the monomer is 49.6 kDa.
Which RNases does Thermo Scientific RiboLock RNase Inhibitor inhibit?

Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A, B, and C. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 or prokaryotic RNases I and H.

For Research Use Only. Not for use in diagnostic procedures.