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Quantifiler™ Human DNA Quantification Kit
Specifications
Specifications
| Content And Storage | This kit contains sufficient reagents for 400 reactions: • Quantifiler PCR Reaction Mix (store at 2 to 8°C). • Quantifiler Human DNA Standard (store at -15 to -25°C). • Quantifiler Human Primer Mix (store at -15 to -25°C). |
| Detection Limit | Can reproducibly detect <16 pg⁄μl of human genomic DNA |
| Detection Method | Primer-Probe Detection |
| Format | Tube |
| Sample Type | DNA (Genomic), Forensic Sample, Forensic Sample (Highly Degraded) |
| For Use With (Equipment) | 7000 System, 7500 System |
| No. of Reactions | 400 Preps |
| PCR Method | qPCR |
| Product Line | Quantifiler |
| Shipping Condition | Dry Ice |
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Frequently Asked Questions (FAQs)
The sizes of the amplicons were chosen to improve the estimation of DNA quantity, thereby delivering a more realistic estimate of the amount of DNA present in degraded samples and providing better correlation between the quantitation values and the performance of the AmpFℓSTR kits.
The "HP" stands for "Human Plus" because this kit is intended as an upgrade to the Quantifiler Human DNA Quantification Kit. Like the Quantifiler Human kit, the new Quantifiler HP kit detects and quantifies human DNA (without a male target), but the new kit also includes a large autosomal (LA) target to enable degradation index functionality and more comprehensive sample quality assessment. It also features increased sensitivity and robustness to better correlate with Applied Biosystems STR chemistries such as those in the Identifiler Plus, NGM SElect, and GlobalFiler PCR Amplification Kits.