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Invitrogen™ PureLink™ Microbiome DNA Purification Kit

Numéro de catalogue. A29790
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50 Preps kit
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Numéro de catalogue. Quantity
A29790 50 Preps kit
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Numéro de catalogue. A29790 Fournisseur Invitrogen™ Code fournisseur A29790
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Description

The Invitrogen™ PureLink™ Microbiome DNA Purification Kit uses proven PureLink™ spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing or other applications.

The Invitrogen™ PureLink™ Microbiome DNA Purification Kit uses proven PureLink™ spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing or other applications. The highly efficient triple lysis approach, fast removal of inhibitors, and versatility makes PureLink the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.

  • Efficient lysis of all microorganisms (including durable species with thicker and more complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
  • Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
  • Streamlined protocols for numerous biological samples
  • Recovery of highly pure DNA compatible with common downstream applications such as qPCR and next-generation sequencing

Certifications

Certificates of Analysis for each lot

Warranty

Product performance guaranteed for 1 year from date of sale

Spécifications

Column Type Spin Column
Content And Storage • 40 mL S1 Lysis Buffer; room temperature
• 5 mL S2 Lysis Enhancer; room temperature
• 12.5 mL S3 Cleanup Buffer; room temperature
• 45 mL S4 Binding Buffer; room temperature
• 13 mL S5 Wash Buffer (concentrated); room temperature
• 5 mL S6 Elution Buffer; room temperature
• 50 PureLink Columns with Collection Tubes; room temperature
• 100 PureLink Collection Tubes; room temperature
• 50 Bead Tubes; room temperature
Isolation Technology Silica Spin Column
Scale Mini
Sample Type Microbial Culture, Saliva, Soil, Stool, Host DNA from Stool, Swabs (Buccal, Vaginal, Skin, Rectal, or Environmental), Cells, Food and Environmental
Elution Volume 50 to 200 μL
Final Product Type Microbial and host DNA
For Use With (Application) PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility Not High-throughput Compatible (Manual)
Quantity 50 Preps kit
Shipping Condition Room Temperature
Test Time Up to 55 min. (Including sample homogenization)
Yield ≤25 μg
Starting Material Amount Buccal/Vaginal/Skin/Rectal/Environmental Swab: 1 swab
Microbial Culture: ≤5 mL
Saliva: ≤2 mL
Soil: ≤2 g
Stool: ≤2 g
Urine: ≤10 mL
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For research use only. Not for use in diagnostic procedures.