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Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit 
The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/μg) plasmid DNA in about 30 minutes.
Supplier: Invitrogen™ A36227
Description
The simple precipitation-free protocol yields up to 0.4 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.
- Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
- Low endotoxin plasmid—the resulting plasmid contains less than 1 EU/μg endotoxins
- High yields—isolate up to 0.4 mg of high-quality plasmid DNA
- Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization
Specifications
| • Resuspension buffer, store at 4°C upon reciept • Lysis buffer, store at room temperature • Precipitation buffer, store at room temperature • Binding buffer, store at room temperature • Wash buffer 1, store at room temperature • Wash buffer 2 (concentrate), store at room temperature • Elution buffer, store at room temperature • Column assemblies, store at room temperature • Syringe filters, store at room temperature • Syringe plungers, store at room temperature • Collection tubes, store at room temperature |
|
| Silica Column | |
| Cloning, PCR | |
| Midi scale input culture of 25-100 mL to obtain yields of up to 200 μg | |
| Plasmid Purification Kit | |
| Room Temperature |
| Kit | |
| Midi | |
| 50 Preps | |
| PureLink | |
| 50 Preps | |
| 30 min. |
For Research Use Only. Not for use in diagnostic procedures.